Usefulness of matrix-assisted laser desorption ionisation–time-of-flight mass spectrometry for identifying clinical Trichosporon isolates J.N. de Almeida.

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Usefulness of matrix-assisted laser desorption ionisation–time-of-flight mass spectrometry for identifying clinical Trichosporon isolates J.N. de Almeida Júnior, D.S.Y. Figueiredo, D. Toubas, G.M.B. Del Negro, A.L. Motta, F. Rossi, J. Guitard, F. Morio, E. Bailly, A. Angoulvant, D. Mazier, G. Benard, C. Hennequin Clinical Microbiology and Infection Volume 20, Issue 8, Pages 784-790 (August 2014) DOI: 10.1111/1469-0691.12502 Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Box-and-whiskers plot of the logscore values obtained using the three extraction procedures tested in this study. Protocol 1: Bruker's recommended protocol for yeast identification. Protocol 2: described by Cassagne et al. [16]. Protocol 3: described by Sendid et al. [21]. * Statistically significant difference (p 0.012). Clinical Microbiology and Infection 2014 20, 784-790DOI: (10.1111/1469-0691.12502) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Distribution of the logscore (LS) values for 68 strains obtained using matrix-assisted laser desorption ionisation–time-of-flight (MALDI–TOF), according to the reference main spectra (MSP) databases that were searched. The dark line represents the best-matched LS values of the correct identification results, whereas the grey line shows the best-matched LS values of the misidentification results. (a) Biotyper 3.0, database 1: several incorrect identifications with LS values below 1.6 (mainly related to the absence of the species in the main spectra (MSP) database) and with LS values above 2.0 (MSPs with incorrect identification); (b) CBS strains, database 2; (c) Biotyper 3.0 and CBS strains, database 3; (d) Biotyper 3.0 and clinical strains, database 4; (e) CBS and clinical strains, database 5; (f) Biotyper 3.0, CBS and clinical strains, database 6. Databases 5 and 6 yielded the best results, but incorrect identifications of Trichosporon dermatis as Trichosporon mucoides with LS values above 2.0 were noted for one and three isolates, respectively. Clinical Microbiology and Infection 2014 20, 784-790DOI: (10.1111/1469-0691.12502) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions