Volume 54, Pages S136-S138 (September 1998)

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Volume 54, Pages S136-S138 (September 1998) Cationic amino acid transporter mRNA expression in rat kidney and liver Anke Burger-Kentischer, Eva Müller, Hanns-Georg Klein, Andreas Schober, Wolfgang Neuhofer, Franz X. Beck Kidney International Volume 54, Pages S136-S138 (September 1998) DOI: 10.1046/j.1523-1755.1998.06726.x Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 1 Reverse transcription polymerase chain reaction for the rat cationic amino acid transporter-2 (r-CAT-2) digoxigenin-probe of RNA from liver. A molecular size marker is shown on the left. Kidney International 1998 54, S136-S138DOI: (10.1046/j.1523-1755.1998.06726.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 2 Northern blot analyses of rat cationic amino acid transporter-2 (r-CAT-2). (A) mRNA levels in kidney [inner medulla (IN), outer medulla (OM), cortex (c)] and liver. Total RNA (20 μg) was size separated on a 1.0% formaldehyde-agarose gel and transferred to a nylon membrane and hybridized with a digoxigenin-labeled r-CAT-2 cDNA probe. (B) Relative abundancy of r-CAT-2 mRNA in liver and kidney. Kidney International 1998 54, S136-S138DOI: (10.1046/j.1523-1755.1998.06726.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 3 Light micrographs showing pattern of nonradioactive in situ hybridization of the digoxygenin-labeled rat cationic amino acid transporter-2 (r-CAT-2) probe in paraffin-embedded sections of rat liver (A) and kidney (B). Abbreviations for liver are: central vein (CV), portal canal (PC). The section passes through some of the sinusoids (S) as they open into the central vein. Abbreviations for the kidney are: inner medulla (IM), outer medulla (OM), and cortex (c). Kidney International 1998 54, S136-S138DOI: (10.1046/j.1523-1755.1998.06726.x) Copyright © 1998 International Society of Nephrology Terms and Conditions