Suzanne M. Morgan, Elizabeth Hodges, Tracey J

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Presentation transcript:

Molecular Analysis of T-Cell Receptor β Genes in Cutaneous T-Cell Lymphoma Reveals Jβ1 Bias Suzanne M. Morgan, Elizabeth Hodges, Tracey J. Mitchell, Susan Harris, Sean J. Whittaker, John L. Smith Journal of Investigative Dermatology Volume 126, Issue 8, Pages 1893-1899 (August 2006) DOI: 10.1038/sj.jid.5700304 Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Fluorescent TCR Genescan analysis. Representative TCRB multiplex Genescan analysis of DNA extracted from skin biopsies from patients with CTCL. (a and b) Demonstrate a polyclonal Gaussian distribution with the TCRB A mix and TCRB mix, respectively, (c and d) demonstrate V–Jβ2 clonal rearrangements in patients 5 and 6, respectively, (e and g–j) demonstrated V–Jβ1 clonal rearrangements in patients 7, 13, 16, 17, and 18, and (f) demonstrates biallelicV–JB1 clonal rearrangements in patient 11. Journal of Investigative Dermatology 2006 126, 1893-1899DOI: (10.1038/sj.jid.5700304) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Detection of clonal TCRG and TCRB gene rearrangements in the skin of CTCL patients by PCR. Journal of Investigative Dermatology 2006 126, 1893-1899DOI: (10.1038/sj.jid.5700304) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Junctional region nucleotide sequences in clonal TGR Vβ–Dβ–Jβ gene rearrangements in CTCL. Journal of Investigative Dermatology 2006 126, 1893-1899DOI: (10.1038/sj.jid.5700304) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Junctional amino-acid sequences in clonal TCR Vβ–Dβ–Jβ gene rearrangements in CTCL. Journal of Investigative Dermatology 2006 126, 1893-1899DOI: (10.1038/sj.jid.5700304) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 TCRB junctional amino-acid sequences of nine clones amplified from the skin lesion of patient 2. Journal of Investigative Dermatology 2006 126, 1893-1899DOI: (10.1038/sj.jid.5700304) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions