Individual demands of human embryos on IVF culture medium: influence on blastocyst development and pregnancy outcome B. Wirleitner, P. Vanderzwalmen,

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Individual demands of human embryos on IVF culture medium: influence on blastocyst development and pregnancy outcome B. Wirleitner, P. Vanderzwalmen, A. Stecher, M.H. Zech, M. Zintz, N.H. Zech Reproductive BioMedicine Online Volume 21, Issue 6, Pages 776-782 (December 2010) DOI: 10.1016/j.rbmo.2010.08.003 Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Flow diagram of the conducted study comparing single versus sequential medium culture. In the ‘‘One medium culture”, all oocytes of a patient were cultured in the same medium whereas in the ‘‘Sibling culture” the oocytes of each patient were randomly split and maintained in both tested culture media. COC=cumulus–oocyte–complex; PN=pronuclei; MII=metaphase II. Reproductive BioMedicine Online 2010 21, 776-782DOI: (10.1016/j.rbmo.2010.08.003) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions