Alexander J. Lakhter, Ravi P. Sahu, Yang Sun, William K

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Chloroquine Promotes Apoptosis in Melanoma Cells by Inhibiting BH3 Domain– Mediated PUMA Degradation Alexander J. Lakhter, Ravi P. Sahu, Yang Sun, William K. Kaufmann, Elliot J. Androphy, Jeffrey B. Travers, Samisubbu R. Naidu Journal of Investigative Dermatology Volume 133, Issue 9, Pages 2247-2254 (September 2013) DOI: 10.1038/jid.2013.56 Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Chloroquine (CQ) promotes apoptosis in melanoma cell lines. Three non-melanoma and five melanoma cell lines were exposed to 50μM CQ; cell viability (a) and apoptosis (b) were measured at indicated time points. (c, d) Wild-type for N-RAS and BRAF or its mutant melanoma cell lines were treated with CQ as in a and b, and the cell viability (c) and apoptotic response (d) to CQ were assessed. Journal of Investigative Dermatology 2013 133, 2247-2254DOI: (10.1038/jid.2013.56) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Chloroquine (CQ) reduces growth of human melanoma cells in mice. (a) NOD-SCID mice were implanted with SK-MEL23 cells, 21 days post implantation; mice were divided into two groups for vehicle and CQ treatment. Tumor volumes of vehicle or CQ-treated mice were measured at indicated days and the growth kinetics of tumor volume are shown on the graph (P=0.0001). (b) Dissected tumors from vehicle and CQ-treated mice were weighed (P≤0.05). Journal of Investigative Dermatology 2013 133, 2247-2254DOI: (10.1038/jid.2013.56) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Chloroquine (CQ) promotes accumulation of p53 upregulated modulator of apoptosis (PUMA) protein in melanoma cells. (a, b) Indicated melanoma cells were exposed to etoposide or different concentrations of CQ and the cell extracts were blotted with PUMA, p53, p21, and actin antibodies. (c) Quantification of PUMA signal intensity from (panels a and b). (d, e) Melanoma cells were treated with etoposide or CQ, and the relative levels of PUMA transcripts were assayed by quantitative PCR. Journal of Investigative Dermatology 2013 133, 2247-2254DOI: (10.1038/jid.2013.56) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Chloroquine (CQ) inhibits degradation of p53 upregulated modulator of apoptosis (PUMA) protein in melanoma cells. (a, b) To determine the half-life of endogenous PUMA protein, control or CQ-treated melanoma cells were exposed to cycloheximide, a protein synthesis inhibitor, and cell extracts collected at different time points were blotted with PUMA antibody. (c) Protein decay analysis from three experiments is shown. Journal of Investigative Dermatology 2013 133, 2247-2254DOI: (10.1038/jid.2013.56) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 The Bcl homology-3 (BH3) domain of p53 upregulated modulator of apoptosis (PUMA) mediates its degradation. (a) To determine the role of the BH3 domain in PUMA protein stability, recombinant plasmids coding for HA-tagged wild-type (wt) or the BH3 domain deletion mutant PUMA were ectopically expressed in melanoma cells, and the cell extracts were blotted with HA antibody or actin antibodies. (b) Densitometry scanning of three experiments is shown on a graph. (c) Schematic showing BH3 domain and its mutant peptides fusion with green fluorescent protein (GFP). (d) Plasmids from c were introduced into melanoma cells and the GFP expression was imaged. (e) Mutant BH3 domain– and wt BH3 domain–fused GFP were expressed in melanoma cells, and the cells were treated with chloroquine (CQ) and blotted with indicated antibodies. Journal of Investigative Dermatology 2013 133, 2247-2254DOI: (10.1038/jid.2013.56) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 6 Lysosomal protease-independent degradation of p53 upregulated modulator of apoptosis (PUMA) protein in melanoma cells. (a, b) Melanoma cells were exposed to chloroquine (CQ) or lysosomal protease inhibitor cocktails (E64d+pepstatin (a) and the cell extracts were blotted with antibodies shown. (c) Melanoma cells were transfected with green fluorescent protein (GFP)–LC3, exposed to CQ or lysosomal protease inhibitor cocktails, and visualized under microscope. (d) GFP punctae were counted from three experiments shown on graph. (e) Melanoma cells were treated with CQ or lysosomal protease inhibitors, and the measured caspase activity is shown. Journal of Investigative Dermatology 2013 133, 2247-2254DOI: (10.1038/jid.2013.56) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions