High-Efficiency Stable Gene Transduction in Primary Human Melanocytes Using a Lentiviral Expression System  Shariff Dunlap, Xiaobing Yu, Linzhao Cheng, Curt I. Civin, Rhoda M. Alani  Journal of Investigative Dermatology  Volume 122, Issue 2, Pages 549-551 (February 2004) DOI: 10.1046/j.0022-202X.2004.22214.x Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Primary human melanocytes maintain high-level expression of transduced genes several weeks after lentiviral infection. (A) Phase contrast and fluorescence images of primary human melanocytes infected with EF. GFP lentivirus 48 h after infection. Note all cells seen phase contrast express GFP. (B) Phase contrast and fluorescence images of primary human melanocytes infected with EF-GFP lentivirus 4 wk after infection. Note all cells seen in phase contrast express GFP. Journal of Investigative Dermatology 2004 122, 549-551DOI: (10.1046/j.0022-202X.2004.22214.x) Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 High-efficiency gene transduction in primary human melanocytes using a bicistronic lentivirus. Phase contrast image (A) and fluorescence microscopy of primary human melanocytes infected with a bicistronic lentivirus expressing GFP (B) and RFP (C) at 24 h after infection. Note expression of GFP in uninfected (–) and infected (+) cells as determined by western analysis 72 h after infection (D). Journal of Investigative Dermatology 2004 122, 549-551DOI: (10.1046/j.0022-202X.2004.22214.x) Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions