Inhibition of protein kinase Cα improves myocardial β-adrenergic receptor signaling and ventricular function in a model of myocardial preservation Karen.

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Presentation transcript:

Inhibition of protein kinase Cα improves myocardial β-adrenergic receptor signaling and ventricular function in a model of myocardial preservation Karen M. D’Souza, PhD, Natalia N. Petrashevskaya, PhD, Walter H. Merrill, MD, Shahab A. Akhter, MD The Journal of Thoracic and Cardiovascular Surgery Volume 135, Issue 1, Pages 172-179.e1 (January 2008) DOI: 10.1016/j.jtcvs.2007.08.035 Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

Figure 1 Creatine kinase activity in 1 mL of myocardial effluent after 90 minutes of normothermic reperfusion. *P < .01 versus control (SA group); n = 9 in each group. The Journal of Thoracic and Cardiovascular Surgery 2008 135, 172-179.e1DOI: (10.1016/j.jtcvs.2007.08.035) Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

Figure 2 Myocardial PKCα activity after cardioplegic arrest, cold preservation, and normothermic reperfusion. M, Membrane fraction; C, cytosolic fraction; CP, cardioplegia (University of Wisconsin) solution. *P < .05 versus control; n = 9 in each group. The Journal of Thoracic and Cardiovascular Surgery 2008 135, 172-179.e1DOI: (10.1016/j.jtcvs.2007.08.035) Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

Figure 3 Myocardial PKCϵ activity after cardioplegic arrest, cold preservation, and normothermic reperfusion. M, Membrane fraction; C, cytosolic fraction; CP, cardioplegia (University of Wisconsin) solution. *P < .05 versus control; n = 7 in each group. The Journal of Thoracic and Cardiovascular Surgery 2008 135, 172-179.e1DOI: (10.1016/j.jtcvs.2007.08.035) Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

Figure 4 G protein–coupled receptor kinase (GRK2) activity before and after cold preservation and normothermic reperfusion. SA, Saline; CP, cardioplegia (University of Wisconsin) solution. *P < .001 versus control (SA group); n = 8 in each group. The Journal of Thoracic and Cardiovascular Surgery 2008 135, 172-179.e1DOI: (10.1016/j.jtcvs.2007.08.035) Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

Figure 5 A, G protein–coupled receptor kinase (GRK2) activity in transgenic protein kinase C (PKC) α hearts with specific inhibition (INH) and PKCα hearts with specific activation (ACT) before and after cold preservation and normothermic reperfusion. B, Baseline; P, after reperfusion; NTg, nontransgenic. *P < .001 versus NTg control and PKCα INH hearts; n = 8 in each group. B, Total myocardial GRK2 protein expression. There was no difference between groups. B, Baseline; P, after reperfusion; NTg, nontransgenic. N = 8 in each group. The Journal of Thoracic and Cardiovascular Surgery 2008 135, 172-179.e1DOI: (10.1016/j.jtcvs.2007.08.035) Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions