pIRS1(S636/639) IRS1 actin PF Supplemental Figure S1

Slides:

Advertisements

Similar presentations

Supplement Figure 1, Pretreatment with GBE has no significant effects on chronic high-glucose- induced AP-1 and NF-  B activation in HEACs. Glucose (25.

Advertisements

Glucose mM Laminin β1 Actin Insulin Laminin β1 Actin 010pM100pM1nM S 1A. Dose-dependent increase in high glucose (HG)- and high insulin (HI)-induced.

Date of download: 6/22/2016 The Association for Research in Vision and Ophthalmology Copyright © All rights reserved. From: Regulation of IGF-I Signaling.

Shimura Supplemental Figure 1

An anti-CD19 antibody inhibits the interaction between P-glycoprotein (P-gp) and CD19, causes P-gp to translocate out of lipid rafts, and chemosensitizes.

Figure 3 The subcellular distribution of LPL activity in 3T3-L1 adipocytes. Adipocytes were made insulin resistant (desensitized) by treatment with 100.

Figure 1. GLP-1 induces miR-132 and miR-212 expressions in pancreatic β-cells in vitro. A, miRNA expression profiling of GLP-1-treated INS-1 832/3 cells.

Figure 4. Effects of hydrogenized water on SIRT1 expression in human umbilical vein endothelial cells. Human umbilical vein endothelial cells were incubated.

Cell Physiol Biochem 2013;32: DOI: /

Supplementary Figure 1 Sensitive Resistant Cell Count Propidium Iodide

Volume 67, Issue 3, Pages (March 2005)

Volume 4, Issue 12, Pages (December 2015)

Supplemental Figures Supplemental Figure 1.

Phosphorylation of RelA Ser311 in vivo.

The Small-Molecule Iron Transport Inhibitor Ferristatin/NSC Promotes Degradation of the Transferrin Receptor Lior Horonchik, Marianne Wessling-Resnick

Jaya Sahni, Andrew M. Scharenberg Cell Metabolism

Resistin decreases insulin-like growth factor I–induced steroid production and insulin- like growth factor I receptor signaling in human granulosa cells

Volume 13, Issue 4, Pages (February 2003)

Epidermal Growth Factor Induces Fibronectin Expression in Human Dermal Fibroblasts via Protein Kinase C δ Signaling Pathway Yoshihiro Mimura, Hironobu.

Identifying Inhibitors of Epithelial-Mesenchymal Transition by Connectivity Map–Based Systems Approach Ajaya Kumar Reka, PhD, Rork Kuick, MS, Himabindu.

A B 20% O2 5% O2 C 5% O2 + NAC DAPI gH2AX pLKO.1 GFP-SOD1 S 0.1Gy

Phosphatidylinositol 3-Kinase/Akt-Dependent and -Independent Protection Against Apoptosis in Normal Human Melanocytes Masahiro Oka, Akiko Kageyama, Mizuho.

Volume 6, Issue 1, Pages (July 2000)

Volume 69, Issue 2, Pages (January 2006)

Metabolic Stress Signaling Mediated by Mixed-Lineage Kinases

relative to untreated vector

Volume 17, Issue 5, Pages (May 2013)

Volume 15, Issue 1, Pages (January 2012)

Volume 8, Issue 4, Pages (August 2014)

Neurotransmitter-Induced Inhibition of Exocytosis in Insulin-Secreting β Cells by Activation of Calcineurin Erik Renström, Wei-Guang Ding, Krister Bokvist,

Volume 18, Issue 6, Pages (December 2013)

IL-15Rα Recycles and Presents IL-15 In trans to Neighboring Cells

Lipotransin Molecular Cell

Stefanie S. Schalm, Diane C. Fingar, David M. Sabatini, John Blenis

Bidirectional Transport of Amino Acids Regulates mTOR and Autophagy

Volume 29, Issue 3, Pages (February 2008)

Volume 70, Issue 9, Pages (November 2006)

Inappropriate Activation of the TSC/Rheb/mTOR/S6K Cassette Induces IRS1/2 Depletion, Insulin Resistance, and Cell Survival Deficiencies O.Jameel Shah,

Volume 12, Issue 1, Pages (July 2015)

Volume 29, Issue 5, Pages (March 2008)

TSC2 regulates VEGF through mTOR-dependent and -independent pathways

Volume 60, Issue 3, Pages (September 2001)

Volume 3, Issue 4, Pages (April 2006)

Volume 6, Issue 4, Pages (October 2007)

Volume 14, Issue 1, Pages (July 2011)

Matrix metalloproteinase-13 influences ERK signalling in articular rabbit chondrocytes L.J. Raggatt, Ph.D., S.C. Jefcoat, M.S., I. Choudhury, Ph.D., S.

Volume 61, Issue 5, Pages (May 2002)

The Actin-Bundling Protein Palladin Is an Akt1-Specific Substrate that Regulates Breast Cancer Cell Migration Y. Rebecca Chin, Alex Toker Molecular.

Reh cells, 24 hours c-Myc Ac-p53 b-actin

Glutaminolysis Activates Rag-mTORC1 Signaling

Involvement of PI3K activation in TCR/CD3-dependent HIF-1α protein expression. Involvement of PI3K activation in TCR/CD3-dependent HIF-1α protein expression.

Rapamycin inhibits TCR/CD3 engagement-dependent HIF-1α protein expression. Rapamycin inhibits TCR/CD3 engagement-dependent HIF-1α protein expression. Human.

Leto et al., Supplementary Figure S3

Volume 17, Issue 1, Pages (January 2010)

Supplementary Figure 1 A B C SW620 HT29 SW620

Angiotensin II stimulates Pax-2 in rat kidney proximal tubular cells: Impact on proliferation and apoptosis Shao-Ling Zhang, Jun Guo, Babak Moini, Julie.

Vehicle Akti 0’ 2’ 5’ 10’ 0’ 2’ 5’ 10’ AktT308 β-actin GSK3βS9 β-actin

Ken Inoki, Masakazu Haneda, Shiro Maeda, Daisuke Koya, Ryuichi Kikkawa

Volume 70, Issue 5, Pages (September 2006)

AMPK directly increases mTORC2 catalytic activity.

P38 activation mediates chronic insulin-induced IRS1 and IRS2 degradation and is involved in myocardial insulin resistance in vitro. p38 activation mediates.

Adipose tissue dysfunction induces increased liver HIF-1α expression

RhoA GTPase Regulates B Cell Receptor Signaling

Chronic rapamycin treatment impairs β-cell mass and insulin clearance in rats. Chronic rapamycin treatment impairs β-cell mass and insulin clearance in.

Insig-mediated degradation of HMG CoA reductase stimulated by lanosterol, an intermediate in the synthesis of cholesterol Bao-Liang Song, Norman B. Javitt,

Supplemental Fig. 1 Fig. S1. Requirement for both ALA treatment and irradiation to elicit a stress response. PC3 cells in serum-free medium were dark-incubated.

GA blocks HIF activity and reduces HIF target expression.

Figure 4. EC-18 dephosphorylated activated STAT6

AG1024 reduced the level of tyrosyl-phosphorylated proteins, including Erk2. AG1024 reduced the level of tyrosyl-phosphorylated proteins, including Erk2.

Volume 11, Issue 5, Pages (May 2010)

Presentation transcript:

- + - + pIRS1(S636/639) IRS1 actin PF4708671 Supplemental Figure S1 Immunoblot analysis. RPE cells were incubated in serum free DMEM (25 mM glucose) in the presence or absence of 10 µM PF 4708671 for 42-44 hours and then were stimulated with 1 µg/ml insulin and lysed.

Rap IR insulin - + - + - + - + - HIF1α actin DMEM MEM o/n chronic IR insulin - + - + - + - + - HIF1α actin DMEM MEM Supplemental Figure S2 Immunoblot analysis. RPE cells were maintained either in DMEM (25 mM glucose) or in MEM (5.5 mM glucose). Cells were incubated in serum-free respective medium for 42-44 hr and then stimulated with 1 µg/ml insulin for 15 min and lysed, Rap – 10 nM rapamycin; o/n – cells were pretreated with rapamycin overnight before stimulation; chronic – cells pre-treated with rapamycin for 2 weeks before stimulation; IR – cells were pre-treated with 1 µg/ml insulin overnight before stimulation.