ANOVA Within Strain P-values

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ANOVA Within Strain P-values Wild-type data had overall more significant gene expression changes. ANOVA WILD-TYPE DCIN5 DGLN3 DHAP4 DSWI4 p < 0.05 2377 (38.41%) 1995 (32.23%) 1856 (30.00%) 2387 (38.57%) 2583 (41.74%) p < 0.01 1531 (24.74%) 1157 (18.69%) 1007 (16.27%) 1489 (24.06%) 1679 (27.13%) p < 0.001 850 (13.73%) 566 (9.15%) 398 (6.43%) 679 (10.97%) 869 (14.04%) p < 0.0001 449 (7.25%) 280 (4.52%) 121 (1.96%) 240 (3.88%) 446 (7.21%) B&H p < 0.05 1673 (31.88%) 1117 (18.05%) 889 (14.36%) 1615 (20.09%) 1855 (29.97) Bonferroni p < 0.05 226 (3.65%) 109 (1.76%) 20 (0.32%) 61 (0.99%) 179 (2.89%)

For the T-test, T60 had the most significant gene expression values; however, T30 had the most significant p-values for the B&H criterion. Cold Shock Recovery t test t15 t30 t60 t90 t120 Average Log Fold Change > 0.25 and p < 0.05 582 (9.4%) 865 (14.0%) 933 (15.1%) 439 (7.1%) 230 (3.7%) Average Log Fold Change < -0.25 and 486 (7.9%) 717 (11.6%) 867 309 (5.0%) 247 (4.0%) Total 1075 (17.4%) 1587 (25.6%) 1814 (29.3%) 749 (12.1%) 509 (8.2%) Total B & H (0%) 85 (1.4%) 1 (0.016%) Total Bonferroni

T-test Comparisons dHAP4 saw an overall higher change within the individual time points than the wild-type resulted. Cold Shock Recovery t test t15 t30 t60 t90 T120 WILD TYPE 1075 (17.4%) 1587 (25.6%) 1814 (29.3%) 749 (12.1%) 509 (8.2%) DCIN5 1393 (22.5%) 756 (12.2%) 1250 (30.2%) 634 (10.2%) 351 (5.6%) DGLN3 1027 (16.6%) 1622 (26.2%) 628 (10.1%) 558 (9.0%) 403 (6.5%) DHAP4 1197 (19.3%) 1772 (28.6%) 2006 (32.4%) 234 (3.7%) 515 (8.3%) DSWI4 N/A 1497 (24.2%) 1757 (28.4%) 705 (11.4%)

Between Strain ANOVA Comparison Results Looking between the two different species, there was more significant differences in the gene expression levels than within the wt and a deletion strain. Comparison p < 0.05 B&H p <0.05 wt vs. dCIN5 563 4 wt vs. dGLN3 720 36 wt vs. dHAP4 640 23 wt vs. dHMO1 556 5 wt vs. ZAP1 553 31 wt vs. S. par 1498 703

There were 5 significant profiles that STEM identified from the statistical analysis of the significant changes in gene expression.

Profile#45 saw up-regulation during cold shock and down regulation during recovery time.

Profile#22 had little to no change in expression during cold shock and up-regulation during recovery time.

Profile#9 saw down-regulation when the yeast was induced to cold shock and up-regulation during recovery time.

Profile#28 was up-regulated during cold shock while its expression level reached its initial state in recovery time.

Profile#48 was up-regulated during cold shock and was approaching its initial state during recovery time

Kittens made from Profile #43