Volume 21, Issue 9, Pages (September 2014)

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Volume 21, Issue 9, Pages 1162-1170 (September 2014) Screening Technologies for Small Molecule Discovery: The State of the Art William P. Janzen Chemistry & Biology Volume 21, Issue 9, Pages 1162-1170 (September 2014) DOI: 10.1016/j.chembiol.2014.07.015 Copyright © 2014 Elsevier Ltd Terms and Conditions

Figure 1 Modular Compound Management Systems (A) Standardized storage vessels. Compounds are stored in standardized barcoded vessels. From left,1. dry compound storage vials for up to gram quantities,2. automation-friendly vials for dissolution and transfer,3. microtubes for storage in automate tubes stores. The carrier is barcoded, and each tube is also tracked with a 2D barcode, and4. barcoded microplates, different formats may be used for storage and assay. (B) Modular storage units. Modular storage units can be arrayed and added or removed as capacity needs change. These units can be isolated and used as independent workstations or connected by a variety of automated compilers that range from robotic arms to conveyer systems to pneumatic tubes. Once a set of compounds is compiled, it is transferred to microplates that can be stored for later use or transferred directly to automated screening systems. Chemistry & Biology 2014 21, 1162-1170DOI: (10.1016/j.chembiol.2014.07.015) Copyright © 2014 Elsevier Ltd Terms and Conditions

Figure 2 Application of Screening Technologies to Cellular or Biochemical Assays Chemistry & Biology 2014 21, 1162-1170DOI: (10.1016/j.chembiol.2014.07.015) Copyright © 2014 Elsevier Ltd Terms and Conditions

Figure 3 Relative Complexity and Degree of Compound Interference for Screening Technologies Complexity is based on the difficulty of assay development, instrumentation required, and operational issues. Compound interference represents how prone that technology is to interference from small molecule compounds in a typical screening library. Chemistry & Biology 2014 21, 1162-1170DOI: (10.1016/j.chembiol.2014.07.015) Copyright © 2014 Elsevier Ltd Terms and Conditions