Fundamentals of Microbiology Biol 315 Lab 2 Instruments and common chemicals in microbiology, Microscopy, Sterilization techniques.

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Presentation transcript:

Fundamentals of Microbiology Biol 315 Lab 2 Instruments and common chemicals in microbiology, Microscopy, Sterilization techniques

Common Instruments Autoclave (121 C, 15 psi) Oven (0-300 C) Incubator (0-120 C) pH Meter Hotplate and Magnetic Stirrer Laminar Flow Cabinet / Biosafety Cabinet

AUTOCLAVE

Autoclave_Portable_Autoclave_Laboratory_Au toclave_Laboratory_Equipments.jpg

instruments/vertical-autoclave.jpg

int.com/upload/image_files/articles/images/co mpanies/865/large-capacity-autoclave-b.jpg

medical/autoclave.jpg

Oven

/1-250x250.jpg

/Hot-Air-Oven---Lab- Model_250x250.jpg

mages/hotairoven.jpg

INCUBATORS

equipment.com/adminimages/Incubators_SI50 5_open.jpg

equipment.com/adminimages/Incubators_SI50 5_closed.jpg

_1.jpg Shaking Incubator

pH Meter

/3505-ph-meter.jpg

Hotplate and magnetic stirrer

scilogex-ms-h-s-m vlarge-r_1.jpg

irrers/adv_hps_lge.jpg

boratories-Magnetic-Stirrer-Hotplate.jpg

Biosafety cabinet

Images/biological_safety_cabinet_purifier_Cov er.jpg

Class I Cabinets These cabinets have unrecirculated airflow away from the operator that is discharged to the atmosphere after filtration through a HEPA filter. They provide good operator protection but do not protect the material within the cabinet (the product) from contamination.

Class II Cabinets Class II cabinets are designed for personnel, product and environmental protection. They are designed for work involving microorganisms in containment levels 2, 3 and 4 laboratories. Class II cabinets are most commonly used in biomedical research laboratories because of their characteristics.

cts-big/ jpg

Class III Cabinets Class III cabinets are totally enclosed and gas- tight with HEPA filtered supply and exhaust air. Work is performed with attached long-sleeved gloves. The cabinet is kept under negative pressure and airflow is maintained by a dedicated exterior exhaust system. Class III cabinets protect the worker and the product. They are designed for work with level 4 pathogens. The exhaust air is double HEPA filtered or treated by HEPA filter and incineration.

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Chemicals Media (solid and liquid) – Nutrient agar, nutrient broth – EMB agar, MacConkeys agar, Blood agar – Lactose broth Grams staining kit Catalase reagent Oxidase reagent

Microscopy Light microscope Photography and video making through microscope using live camera Other kinds of microscopy

Grams Staining result

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THANK YOU