Electromechanical characterization of a tissue-engineered myocardial patch derived from extracellular matrix Takeyoshi Ota, MD, PhD, Thomas W. Gilbert,

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Presentation transcript:

Electromechanical characterization of a tissue-engineered myocardial patch derived from extracellular matrix Takeyoshi Ota, MD, PhD, Thomas W. Gilbert, PhD, Stephen F. Badylak, DVM, MD, PhD, David Schwartzman, MD, Marco A. Zenati, MD The Journal of Thoracic and Cardiovascular Surgery Volume 133, Issue 4, Pages 979-985 (April 2007) DOI: 10.1016/j.jtcvs.2006.11.035 Copyright © 2007 The American Association for Thoracic Surgery Terms and Conditions

Figure 1 A, Intraoperative view. The tissue-engineered patch is placed on the right ventricular wall. B, No infection and aneurysm are noted on the patch 60 days after implantation (arrow). C, Endocardial side of the patch. White tissue is observed. There is no thrombus in the right ventricle. D, Cross section of the patch. The patch region is as thick as the adjoining right ventricular wall. The Journal of Thoracic and Cardiovascular Surgery 2007 133, 979-985DOI: (10.1016/j.jtcvs.2006.11.035) Copyright © 2007 The American Association for Thoracic Surgery Terms and Conditions

Figure 2 Histologic preparations of the patch 60 days after implantation. A, A good repopulation of cells was observed. B, There was some vasculogenesis in the middle of the patch. C, A monolayer of endothelial cells covered the surface of the tissue. D, Multilayered myocytes were observed beneath the monolayer cells. E, Central part of the patch. Regenerated cells were prone to be unevenly distributed in the endocardial aspect at the around center of the patch. F, Marginal part of the patch. The myocytes extended across full thickness at the edge of the patch. (A and B, hematoxylin–eosin stain; C, factor VIII stain; D, α-smooth muscle actin stain; E and F, Masson trichrome stain. (Magnifications A, B, C, and D ×100; E and F ×40.) The Journal of Thoracic and Cardiovascular Surgery 2007 133, 979-985DOI: (10.1016/j.jtcvs.2006.11.035) Copyright © 2007 The American Association for Thoracic Surgery Terms and Conditions

Figure 3 Color-coded electrical map using NOGA. Bipolar electrogram amplitude map. Red demarcates peak-to-peak amplitude < 0.5 mV; violet > 5.0 mV. The Journal of Thoracic and Cardiovascular Surgery 2007 133, 979-985DOI: (10.1016/j.jtcvs.2006.11.035) Copyright © 2007 The American Association for Thoracic Surgery Terms and Conditions

Figure 4 A, Electrogram amplitude graph. Central zone corresponds to the area displayed in red in Figure 3. Marginal zone corresponds to yellow and green. Normal zone corresponds to violet. B, A graph of the linear local shortening. LLS, Linear local shortening; UBM, urinary bladder matrix. The Journal of Thoracic and Cardiovascular Surgery 2007 133, 979-985DOI: (10.1016/j.jtcvs.2006.11.035) Copyright © 2007 The American Association for Thoracic Surgery Terms and Conditions

Figure 5 Overlapped figure standardized the scale (white bar = 1.25 cm). The patch region includes the marginal zone displayed in yellow and green. The Journal of Thoracic and Cardiovascular Surgery 2007 133, 979-985DOI: (10.1016/j.jtcvs.2006.11.035) Copyright © 2007 The American Association for Thoracic Surgery Terms and Conditions