Mutations in CYC1, Encoding Cytochrome c1 Subunit of Respiratory Chain Complex III, Cause Insulin-Responsive Hyperglycemia Pauline Gaignard, Minal Menezes,

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Mutations in CYC1, Encoding Cytochrome c1 Subunit of Respiratory Chain Complex III, Cause Insulin-Responsive Hyperglycemia Pauline Gaignard, Minal Menezes, Manuel Schiff, Aurélien Bayot, Malgorzata Rak, Hélène Ogier de Baulny, Chen-Hsien Su, Mylene Gilleron, Anne Lombes, Heni Abida, Alexander Tzagoloff, Lisa Riley, Sandra T. Cooper, Kym Mina, Padma Sivadorai, Mark R. Davis, Richard J.N. Allcock, Nina Kresoje, Nigel G. Laing, David R. Thorburn, Abdelhamid Slama, John Christodoulou, Pierre Rustin The American Journal of Human Genetics Volume 93, Issue 2, Pages 384-389 (August 2013) DOI: 10.1016/j.ajhg.2013.06.015 Copyright © 2013 The American Society of Human Genetics Terms and Conditions

Figure 1 Gene Analysis in Families Affected by CYC1 Mutations (A) Pedigrees of the Lebanese (with P1) and Sri Lankan (with P2) families. Affected individuals (dark symbols) harbor homozygous (−/−) mutations. Unaffected individuals are either heterozygous (−/+) or wild-type (+/+). (B) Analysis of CYC1 genomic DNA. For P1, a single candidate disease-causing homozygous missense variant in CYC1 was identified by exome sequencing. For P2, who has defective CIII activity, a candidate-gene strategy resulted in sequencing cDNA obtained from total RNA of cultured fibroblasts and revealed a missense mutation (c.643C>T) in CYC1. (C and D) Cyt c1 structures (C) and alignment (D). The American Journal of Human Genetics 2013 93, 384-389DOI: (10.1016/j.ajhg.2013.06.015) Copyright © 2013 The American Society of Human Genetics Terms and Conditions

Figure 2 Analysis of Cyt c1 Mutant Cells and Rescue in Yeast Cells (A–C) Immunoblots of affected individuals’ (P1 [with Cyt c1 p.Trp96Cys] and P2 [with Cyt c1 p.Leu215Phe]) RCs under denaturing (A and B) or native (C) conditions. (A and B) Immunoblot of the levels of Cyt c1 oxidative-phosphoylation subunits in fibroblasts and skeletal muscle (A and B). Compared to those of the controls (Ctrl1–Ctrl4), levels of Cyt c1 and CIII were severely reduced in affected individual fibroblasts and skeletal muscle. Secondary reductions of assembly-dependent subunits of CI and CIV were also observed. SDS-PAGE and immunoblot were carried out on whole-cell lysate from fibroblasts (20 μg) or skeletal-muscle homogenate (5 μg). “¼” indicates that ¼ of the sample was loaded. (C) Protein extract from fibroblasts (6% digitonin; 50 μg) was separated by blue-native PAGE on a 3.5%–12% polyacrylamide gel. Proteins were transferred to a polyvinylidene fluoride (PVDF) membrane and probed with a set of antibodies as indicated in the figure. The various supercomplexes and complexes and the ATP synthase (F1F0) are identified in the margins. (D) Growth of wild-type and mutant yeast. Serial dilutions of the parental W303-1A strain, the Δy-cyc1 null mutant, and the null mutant harboring the wild-type (Δy-cyc1/i-yCYC1) or mutant genes in a single copy by integration at the URA3 locus (Δy-cyc1/i-y-cyc1) or in multiple copies in an episomal plasmid (Δy-cyc1/e-y-cyc1) were serially diluted and spotted on plates supplemented with glucose or glycerol plus ethanol and incubated at 30°C for 2 days. (E) Immunoblot analysis of yeast Cyt c1. In the upper two panels, mitochondrial extracts (3% digitonin; 250 μg protein) were separated by blue-native PAGE on a 4%–13% polyacrylamide gel. Proteins were transferred to a PVDF membrane and stained with Ponceau red before being probed with a polyclonal antibody against yeast Cyt c1. The various supercomplexes and complexes and the ATP synthase (F1F0) are identified in the margins. In the lower panel, mitochondrial proteins (37 μg) were separated by SDS-PAGE on a 15% polyacrylamide gel and transferred to nitrocellulose and probed with a polyclonal antibody against yeast Cyt c1. The American Journal of Human Genetics 2013 93, 384-389DOI: (10.1016/j.ajhg.2013.06.015) Copyright © 2013 The American Society of Human Genetics Terms and Conditions