Ascending aorta dilation in association with bicuspid aortic valve: A maturation defect of the aortic wall Nimrat Grewal, MD, Adriana C. Gittenberger-de.

Slides:

Advertisements

Similar presentations

Of mice and men and surgical transcatheter aortic valve insertion

Advertisements

Mesenchymal stem cells attenuate angiotensin II-induced aortic aneurysm growth in apolipoprotein E-deficient mice Ryotaro Hashizume, MD, Aika Yamawaki-Ogata,

Accelerated aneurysmal dilation associated with apoptosis and inflammation in a newly developed calcium phosphate rodent abdominal aortic aneurysm model

Elastic fibers reconstructed using adenovirus-mediated expression of tropoelastin and tested in the elastase model of abdominal aortic aneurysm in rats

Salusin-β contributes to vascular inflammation associated with pulmonary arterial hypertension in rats Tao Xu, MS, Zhifei Zhang, MD, PhD, Ting Liu, MBBS,

S100A12 Induced in the Epidermis by Reduced Hydration Activates Dermal Fibroblasts and Causes Dermal Fibrosis Jingling Zhao, Aimei Zhong, Emily E. Friedrich,

Pulmonary artery remodeling in transposition of the great arteries: relevance for neoaortic root dilatation Shirin Lalezari, MD, Mark G Hazekamp, MD,

Fariba Chalajour, MD, Laura A

Reconstruction of pulmonary artery with porcine small intestinal submucosa in a lamb surgical model: Viability and growth potential Lorenzo Boni, MD,

Hans-Hinrich Sievers, MD

Effects of azithromycin and tanomastat on experimental bronchiolitis obliterans Katharina Krenn, MD, Matthias Gmeiner, MD, PhD, Patrick Paulus, MD, Nezir.

A proteomic study of the aortic media in human thoracic aortic dissection: Implication for oxidative stress Mingfang Liao, MD, PhD, Zhaoyang Liu, PhD,

Experimental generation of a tissue-engineered functional and vascularized trachea Thorsten Walles, MD, Bettina Giere, Michael Hofmann, PhD, Johanna Schanz,

Gorav Ailawadi, MD, Christopher W. Moehle, BS, Hong Pei, MD, Sandra P

Inflammation-related induction of absent in melanoma 2 (AIM2) in vascular cells and atherosclerotic lesions suggests a role in vascular pathogenesis

Histologic analysis of stent graft oversizing in the thoracic aorta

Association of smooth muscle cell phenotypes with extracellular matrix disorders in thoracic aortic dissection Lixin Wang, MD, PhD, Jing Zhang, MD, PhD,

Dissection of a dilated autograft root

Karen M. Newman, PhD, Jessie Jean-Claude, MD, Hong Li, MS, John V

Michael G. Katz, MD, PhD, Elizabeth Brandon-Warner, PhD, Anthony S

Increased connexin43 expression in human saphenous veins in culture is associated with intimal hyperplasia Sébastien Déglise, MD, David Martin, MS, Hervé.

Pioglitazone preserves vein graft integrity in a rat aortic interposition model Zhi Chen, MD, Tomomi Hasegawa, MD, PhD, Akiko Tanaka, MD, Yutaka Okita,

Hydrogen ameliorates pulmonary hypertension in rats by anti-inflammatory and antioxidant effects Yasuaki Kishimoto, MD, Taichi Kato, MD, PhD, Mikako.

New ascending aortic aneurysm model in rats reproduces main structural features of degenerative ascending thoracic aortic aneurysms in human beings Narcis.

Bone morphogenic protein 2 induces Runx2 and osteopontin expression in human aortic valve interstitial cells: Role of Smad1 and extracellular signal-regulated.

Myocardial regeneration for chronic heart failure: Not as easy as it sounds Richard A. Hopkins, MD The Journal of Thoracic and Cardiovascular Surgery

Ioannis K. Toumpoulis, MD, Julia Thom Oxford, PhD, Douglas B

Effect of microRNA-145 to prevent vein graft disease in rabbits by regulation of smooth muscle cell phenotype Motoaki Ohnaka, MD, Akira Marui, MD, PhD,

Induction of human aortic myofibroblast-mediated extracellular matrix dysregulation: A potential mechanism of fluoroquinolone-associated aortopathy David.

Tissue-engineered, hydrogel-based endothelial progenitor cell therapy robustly revascularizes ischemic myocardium and preserves ventricular function

Transcriptomic analysis of patients with tetralogy of Fallot reveals the effect of chronic hypoxia on myocardial gene expression Mohamed T. Ghorbel,

Vincent L. Rowe, MD, Scott L. Stevens, MD, FACS, Tonya T

Energy loss, a novel biomechanical parameter, correlates with aortic aneurysm size and histopathologic findings Jennifer Chung, MD, Kevin Lachapelle,

Spatiotemporal expression and localization of matrix metalloproteinas-9 in a murine model of thoracic aortic aneurysm Jeffrey A. Jones, PhD, John R.

Bicuspid aortic valve aortopathy: One size fits all?

Recombinant human hepatocyte growth factor transfection alleviates hyperkinetic pulmonary artery hypertension in rabbit models Wei Wang, MD, Kai Liu,

Paul H. Schoof, MDa, Adriana C

Increased macrophage infiltration and neovascularization in congenital bicuspid aortic valve stenosis Pedro R. Moreno, MD, Luis Astudillo, MD, Sammy.

Histologic abnormalities of the ascending aorta and pulmonary trunk in patients with bicuspid aortic valve disease: Clinical relevance to the ross procedure

Role of hemodynamic forces in the ex vivo arterialization of human saphenous veins Xavier Berard, MD, PhD, Sébastien Déglise, MD, Florian Alonso, PhD,

Ascending aortic pseudoaneurysm after aortic valve replacement: Watch the tip of the cardioplegia cannula! Abbas Emaminia, MD, Ahmad Ali Amirghofran,

Three-dimensional evaluation of ductal tissue in coarctation of the aorta using X-ray phase-contrast tomography Ryuma Iwaki, MD, Hironori Matsuhisa,

Pulmonary autograft valve explants show typical degeneration

Stem Cells in Thoracic Aortic Aneurysms and Dissections: Potential Contributors to Aortic Repair Ying H. Shen, MD, PhD, Xiaoqing Hu, MD, Sili Zou, MD,

Reduction of four-and-a-half LIM-protein 2 expression occurs in human left ventricular failure and leads to altered localization and reduced activity.

Giant bronchogenic cyst within the aortic wall mimicking symptoms of acute type A aortic dissection Ömer Senbaklavaci, MD, Lars Oliver Conzelmann, MD,

Distinct macrophage phenotype and collagen organization within the intraluminal thrombus of abdominal aortic aneurysm Jayashree Rao, MS, Bryan N. Brown,

Innovation and science: The future of valve design

Losartan ameliorates “upstream” pulmonary vein vasculopathy in a piglet model of pulmonary vein stenosis Jiaquan Zhu, MD, PhD, Haruki Ide, MD, Yaqin.

Hans-Hinrich Sievers, MD

Characterization of the inflammatory and apoptotic cells in the aortas of patients with ascending thoracic aortic aneurysms and dissections Rumin He,

Photodynamic therapy of vein grafts: Suppression of intimal hyperplasia of the vein graft but not the anastomosis Glenn M. LaMuraglia, MD, Michael L.

In vitro differences between smooth muscle cells derived from varicose veins and normal veins Ying Xiao, PhD, Zhibin Huang, MD, Henghui Yin, PhD, Ying.

A fusion protein of hepatocyte growth factor enhances reconstruction of myocardium in a cardiac patch derived from porcine urinary bladder matrix Takeyoshi.

Layered implantation of myoblast sheets attenuates adverse cardiac remodeling of the infarcted heart Naosumi Sekiya, MD, Goro Matsumiya, MD, PhD, Shigeru.

Restoration of left ventricular geometry and improvement of left ventricular function in a rodent model of chronic ischemic cardiomyopathy Jiashing Yu,

Decellularization reduces calcification while improving both durability and 1-year functional results of pulmonary homograft valves in juvenile sheep

Fenton H. McCarthy, MD, MS, Nimesh D. Desai, MD, PhD

A fate worse than death Jennifer S. Lawton, MD

Shirin Lalezari, MD, Edris A. F. Mahtab, PhD, Margot M

Concomitant replacement of the ascending aorta is free—for some

Richard L. Binns, MD Journal of Vascular Surgery

Identification and characterization of microRNAs in vascular smooth muscle cells from patients with abdominal aortic aneurysms Bernice Lai Yee Cheuk,

Phillip J. Cozzi, B. S. , Ross T. Lyon, M. D. , Harry R. Davis, Ph. D

Discussion The Journal of Thoracic and Cardiovascular Surgery

Matrix metalloproteinase 14 and 19 expression is associated with thoracic aortic aneurysms Veronica Jackson, MD, Therese Olsson, BMA, Sanela Kurtovic,

Toward a more rational approach in treating type B aortic dissection

Inflammatory ascending aortic disease: Perspectives from pathology

Respect the aorta The Journal of Thoracic and Cardiovascular Surgery

Endoluminal smooth muscle cell seeding limits intimal hyperplasia

Presentation transcript:

Ascending aorta dilation in association with bicuspid aortic valve: A maturation defect of the aortic wall Nimrat Grewal, MD, Adriana C. Gittenberger-de Groot, PhD, Robert E. Poelmann, PhD, Robert J.M. Klautz, MD, PhD, Johannes H.N. Lindeman, MD, PhD, Marie-José Goumans, PhD, Meindert Palmen, MD, PhD, Salah A. Mohamed, PhD, Hans-Hinrich Sievers, MD, Ad J.J.C. Bogers, MD, PhD, Marco C. DeRuiter, PhD The Journal of Thoracic and Cardiovascular Surgery Volume 148, Issue 4, Pages 1583-1590 (October 2014) DOI: 10.1016/j.jtcvs.2014.01.027 Copyright © 2014 The American Association for Thoracic Surgery Terms and Conditions

Figure 1 Transverse sections of the aortic wall (A-N). The aorta consists of an intima (i), media (m), and adventitia (a) indicated in A and H. The media and intima are separated by the internal elastic lamella (arrows in B and I). A very compact layering of medial elastic lamellae is seen in TA (C) compared with the somewhat more loosely organized yet regular lamellae in BA (J). The latter is maintained in the BAD group (L), in contrast to the TAD where the lamellae become fragmented and disorganized after dilation (E). Medial thickness is significantly increased in BA and BAD compared with TA and TAD (P). The reverse is seen for the intimal thickness, which is significantly thinner in BA and BAD (O). αSMA staining (G, N) exhibits more severe cystic medial necrosis (CN) in TAD compared with BAD (G). Periaortic inflammation (box in D, indexed as 6) is significantly more severe in TAD compared with BAD (D, F, K, M, and Q). TA, Tricuspid valve, without dilation; TAD, tricuspid valve, with dilation; BA, bicuspid valve, without dilation; BAD, bicuspid valve, with dilation; RF, resorcin fuchsin; HE, hematoxylin-eosin; αSMA, α smooth muscle actin. Scale bars: A, D, G, H, K, and N, 500 μm; B, C, E, F, I, J, L, and M, 50 μm. The Journal of Thoracic and Cardiovascular Surgery 2014 148, 1583-1590DOI: (10.1016/j.jtcvs.2014.01.027) Copyright © 2014 The American Association for Thoracic Surgery Terms and Conditions

Figure 2 Transverse sections of the aortic wall (A, B, D, E, G, H, J, K). Cytoplasmatic 3,3′-diaminobenzidine tetrachloride vascular smooth muscle cell (VSMC) staining, with positivity depicted by an arrow in A, D, and G. The number of αSMA expressing VSMCs is significantly lower in BA (B, indexed as 0) than TA (A, indexed as 6), which is maintained after dilation (BAD and TAD, respectively) (C). The number of SM22α expressing VSMCs is also significantly lower in BA (E, indexed as 2) than TA (D, indexed as 6) and in BAD compared with TAD (F). VSMCs expressing smoothelin were only seen in the TA, mainly in the outer (om) compared with the middle (mm) and inner media (im) (G, indexed as 4) and nearly absent in the other groups (H, J, and K, indexed as 0) (I). TA, Tricuspid valve, without dilation; TAD, tricuspid valve, with dilation; BA, bicuspid valve, without dilation; BAD, bicuspid valve, with dilation; αSMA, α smooth muscle cell actin; SM22α, smooth muscle cell 22α. Scale bars: 200 μm. The Journal of Thoracic and Cardiovascular Surgery 2014 148, 1583-1590DOI: (10.1016/j.jtcvs.2014.01.027) Copyright © 2014 The American Association for Thoracic Surgery Terms and Conditions

Figure 3 Transverse histologic sections of the aortic wall (A-F). Nuclear 3,3′-diaminobenzidine tetrachloride (DAB) staining of lamin A/C (A, brown, see arrow; E, absent staining, see arrow). The number of lamin A/C−expressing nuclei is significantly lower in the BA group compared with the TA group (G). Nuclear DAB staining of progerin is shown in C (brown, see arrow) and F (absent staining, see arrow). The number of progerin-expressing nuclei significantly increases with wall dilation in TAD (H), which is not seen in BAD. The number of progerin-expressing nuclei is significantly lower in BAD compared with TAD (H). Confocal images of consecutive sections (B and D). Nuclei are counterstained with diamidino phenylindole (B and D, blue). Immunofluorescence shows nuclear distribution of lamin A/C (red, B) and nuclear envelope localization of progerin (green, D). TA, Tricuspid valve, without dilation; TAD, tricuspid valve, with dilation; BA, bicuspid valve, without dilation; BAD, bicuspid valve, with dilation. Scale bars: A, C, E, and F, 20 μm; B and D, 1 μm. The Journal of Thoracic and Cardiovascular Surgery 2014 148, 1583-1590DOI: (10.1016/j.jtcvs.2014.01.027) Copyright © 2014 The American Association for Thoracic Surgery Terms and Conditions

Figure 4 Western blot of cell lysate from 8 samples of nondilated aortic wall with a bicuspid aortic valve (BA, 1-4) and tricuspid valve (TA, 5-8) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) used as an internal control sample (Rest). The lamin A/C protein level in each sample was determined by densitometry, performed using ImageJ software, and normalized. The band intensity of the Rest was used as calibrator. The Coomassie Blue stained gel showed equal loading of proteins with a molecular mass of 69 kDa (lamin A), and 62 kDa (lamin C). Bar diagram in white, lamin A level in %; and in black, lamin C level in %. The Journal of Thoracic and Cardiovascular Surgery 2014 148, 1583-1590DOI: (10.1016/j.jtcvs.2014.01.027) Copyright © 2014 The American Association for Thoracic Surgery Terms and Conditions