High prevalence of ST-78 infection-associated vancomycin-resistant Enterococcus faecium from hospitals in Asunción, Paraguay M.A. Khan, J.B. Northwood,

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High prevalence of ST-78 infection-associated vancomycin-resistant Enterococcus faecium from hospitals in Asunción, Paraguay M.A. Khan, J.B. Northwood, R.G.J. Loor, A.T.R. Tholen, E. Riera, M. Falcón, Paraguayan Antimicrobial Network, A. van Belkum, M. van Westreenen, J.P. Hays Clinical Microbiology and Infection Volume 16, Issue 6, Pages 624-627 (June 2010) DOI: 10.1111/j.1469-0691.2009.02898.x Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Pulsed-field gel electrophoresis (PFGE) patterns obtained from 40 infection-associated vancomycin-resistant Enterococcus faecium isolates obtained from Paraguay between 2005 and 2007. Cluster analysis was performed using the method of DICE with UPGMA with band tolerance set to 1.5%. Clinical Microbiology and Infection 2010 16, 624-627DOI: (10.1111/j.1469-0691.2009.02898.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 PCR and sequence-based characterization of Tn1546 in 40 infection-related Enterococcus faecium isolates from Paraguay. Tn1546 reference sequence BM4147 is represented by the unbroken black lines. The position of different genes and open reading frames (orf1 and orf2) are shown by filled block arrows. IS elements are shown by filled boxes and their direction of transcription is represented by arrows. Deletions are represented by dotted lines. The positions of the first nucleotides upstream and downstream of the insertion sequences are shown. The groups are based on the published results of Willems et al. [15]. *A novel F1 variant of Tn1546. Numbers on the left indicate how many isolates were found to belong to each Tn1546 variant. Clinical Microbiology and Infection 2010 16, 624-627DOI: (10.1111/j.1469-0691.2009.02898.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions