MB&B 208: Molecular Biology

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Presentation transcript:

MB&B 208: Molecular Biology Fall 2006 MWF, 10-11 Hall-Atwater 84 http://sholmes.web.wesleyan.edu/wescourses/2006f/mbb208/01/

The “Central Dogma” of Molecular Biology

Gene definition: Classical genetics: The functional unit of inheritance. Molecular genetics: The nucleic acid sequence that is necessary for the synthesis of a functional polypeptide (protein) or RNA.

Transcription initiation in eukaryotes RNA polymerase: CTD = carboxy terminal domain GTF’s = general transcription factors TFIID: includes TBP, binds TATA box TFIIH: helicase and CTD kinase Initiation: phosphorylation of CTD by TFIIH GTF’s do not remain associated with transcribing polymerase

TBP (TATA binding protein)

Gene transcription: role of transcription factors 1. Recruitment of RNA polymerase 2. Overcome inhibition by chromatin structure

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