Volume 8, Issue 6, Pages (December 2001)

Slides:

Advertisements

Similar presentations

Elena Conti, Nick P Franks, Peter Brick Structure

Advertisements

Conservation of Structure and Mechanism between Eukaryotic Topoisomerase I and Site-Specific Recombinases Chonghui Cheng, Paul Kussie, Nikola Pavletich,

Volume 8, Issue 12, Pages (December 2000)

Volume 13, Issue 6, Pages (March 2004)

Volume 8, Issue 6, Pages (December 2001)

Volume 8, Issue 3, Pages (September 2001)

Volume 8, Issue 3, Pages (March 2000)

Crystallographic Structure of SurA, a Molecular Chaperone that Facilitates Folding of Outer Membrane Porins Eduard Bitto, David B. McKay Structure

The Structure of the Cytoplasmic Domain of the Chloride Channel ClC-Ka Reveals a Conserved Interaction Interface Sandra Markovic, Raimund Dutzler Structure

Volume 6, Issue 5, Pages (November 2000)

Volume 124, Issue 1, Pages (January 2006)

Volume 9, Issue 5, Pages (May 2001)

Volume 11, Issue 12, Pages (December 2003)

The crystal structure of Cys-tRNACys–EF-Tu–GDPNP reveals general and specific features in the ternary complex and in tRNA Poul Nissen, Søren Thirup,

Volume 11, Issue 1, Pages (January 2003)

Tom Huxford, De-Bin Huang, Shiva Malek, Gourisankar Ghosh Cell

Crystal Structure of the Soluble Form of Equinatoxin II, a Pore-Forming Toxin from the Sea Anemone Actinia equina Alekos Athanasiadis, Gregor Anderluh,

Tamas Yelland, Snezana Djordjevic Structure

Intramolecular interactions of the regulatory domains of the Bcr–Abl kinase reveal a novel control mechanism Hyun-Joo Nam, Wayne G Haser, Thomas M Roberts,

The Gemin6-Gemin7 Heterodimer from the Survival of Motor Neurons Complex Has an Sm Protein-like Structure Yingli Ma, Josée Dostie, Gideon Dreyfuss, Gregory.

David R Buckler, Yuchen Zhou, Ann M Stock Structure

Volume 20, Issue 6, Pages (December 2005)

Volume 6, Issue 12, Pages (December 1998)

Crystal Structures of Ral-GppNHp and Ral-GDP Reveal Two Binding Sites that Are Also Present in Ras and Rap Nathan I. Nicely, Justin Kosak, Vesna de Serrano,

Crystal Structure of the MHC Class I Homolog MIC-A, a γδ T Cell Ligand

Volume 14, Issue 10, Pages (October 2006)

Crystal Structure of the Human High-Affinity IgE Receptor

N Khazanovich, KS Bateman, M Chernaia, M Michalak, MNG James Structure

Volume 4, Issue 5, Pages (November 1999)

The crystal structure of Cys-tRNACys–EF-Tu–GDPNP reveals general and specific features in the ternary complex and in tRNA Poul Nissen, Søren Thirup,

The 1.9 Å Structure of α-N-Acetylgalactosaminidase

Volume 25, Issue 4, Pages (February 2007)

Volume 124, Issue 5, Pages (March 2006)

Core Structure of gp41 from the HIV Envelope Glycoprotein

Structure of the Cathelicidin Motif of Protegrin-3 Precursor

Danny N.P Doan, Terje Dokland Structure

Moosa Mohammadi, Joseph Schlessinger, Stevan R Hubbard Cell

Andrew H. Huber, W.James Nelson, William I. Weis Cell

Volume 90, Issue 1, Pages (July 1997)

Daniel Peisach, Patricia Gee, Claudia Kent, Zhaohui Xu Structure

Structure and Mechanism of Yeast RNA Triphosphatase

Volume 14, Issue 2, Pages (April 2004)

Volume 100, Issue 3, Pages (February 2000)

Volume 12, Issue 7, Pages (July 2004)

Volume 3, Issue 5, Pages (May 1999)

Antonina Roll-Mecak, Chune Cao, Thomas E. Dever, Stephen K. Burley

Crystallographic Analysis of the Recognition of a Nuclear Localization Signal by the Nuclear Import Factor Karyopherin α Elena Conti, Marc Uy, Lore Leighton,

Volume 6, Issue 6, Pages (December 2000)

Volume 8, Issue 5, Pages (November 2001)

Crystal Structure of a Phosphoinositide Phosphatase, MTMR2

Structural Basis of Rab Effector Specificity

Volume 123, Issue 7, Pages (December 2005)

Solution Structure of a TBP–TAFII230 Complex

NSF N-Terminal Domain Crystal Structure

Structural Role of the Vps4-Vta1 Interface in ESCRT-III Recycling

Crystal Structure of the Tyrosine Phosphatase SHP-2

Crystal Structure of the Flagellar σ/Anti-σ Complex σ28/FlgM Reveals an Intact σ Factor in an Inactive Conformation Margareta K. Sorenson, Soumya S.

Pingwei Li, Gerry McDermott, Roland K. Strong Immunity

Luc Bousset, Hassan Belrhali, Joël Janin, Ronald Melki, Solange Morera

Volume 100, Issue 3, Pages (February 2000)

Structure of the Histone Acetyltransferase Hat1

Crystal Structure of the Human Neuropilin-1 b1 Domain

Structure of E. coli 5′-methylthioadenosine/S-adenosylhomocysteine Nucleosidase Reveals Similarity to the Purine Nucleoside Phosphorylases Jeffrey E.

The Structure of T. aquaticus DNA Polymerase III Is Distinct from Eukaryotic Replicative DNA Polymerases Scott Bailey, Richard A. Wing, Thomas A. Steitz

Crystal Structure of Hyaluronidase, a Major Allergen of Bee Venom

The Crystal Structure of an Unusual Processivity Factor, Herpes Simplex Virus UL42, Bound to the C Terminus of Its Cognate Polymerase Harmon J Zuccola,

Volume 11, Issue 10, Pages (October 2003)

Crystal Structure of Escherichia coli RNase D, an Exoribonuclease Involved in Structured RNA Processing Yuhong Zuo, Yong Wang, Arun Malhotra Structure

Volume 13, Issue 6, Pages (March 2004)

Volume 95, Issue 2, Pages (October 1998)

Presentation transcript:

Volume 8, Issue 6, Pages 1375-1382 (December 2001) Bacterial Polypeptide Release Factor RF2 Is Structurally Distinct from Eukaryotic eRF1 Bente Vestergaard, Lan Bich Van, Gregers R Andersen, Jens Nyborg, Richard H Buckingham, Morten Kjeldgaard Molecular Cell Volume 8, Issue 6, Pages 1375-1382 (December 2001) DOI: 10.1016/S1097-2765(01)00415-4

Figure 1 Structural Presentation of E. coli RF2 (A) Electron density, contoured at 1.8σ, showing the central β sheet of domain 2. Experimental map calculated from data from crystal #2 (left) and final 3Fo − 2Fc σA weighted map (right). The refined model of E. coli RF2 is shown in sticks. (B) Cartoon presentation of E. coli RF2. On the right, the molecule is rotated 90° around the vertical axis with respect to the orientation on the left. The functionally important SPF and GGQ motifs are exposed on the curved surface of the molecule. Domain 1 is shown in orange, domain 2 is in blue, domain 3 is in magenta, and domain 4 is in green. The figure was prepared using MolScript and Raster3D. Molecular Cell 2001 8, 1375-1382DOI: (10.1016/S1097-2765(01)00415-4)

Figure 2 Conserved residues in E. coli RF2 (A) Surface (left) and snake (right) presentation of E. coli RF2. Residues are colored according to the degree of conservation among bacterial RF2, ranging from green (low) to red (high). The asterisk marks a conserved area across the β sheet of domains 2 and 4. Trp47, the SPF motif, and the GGQ motif are marked. The surface representation was made using GRASP and color-coded according to a sequence alignment produced by the ClustalW program. (B) Alignment of E. coli RF2 residues 56–138 in domain 1 with bacterial RF2 (top) and RF1 (bottom). Above the observed secondary structure of RF2 and a computer-generated predicted secondary structure of RF1 is depicted. “H” signifies α helix, “E” indicates β strand, and “C” indicates coil. Residue numbers above the conserved motifs correspond to E. coli RF2 numbering. Below, a bar marks the borders of structurally conserved and structurally distinct areas, respectively. Molecular Cell 2001 8, 1375-1382DOI: (10.1016/S1097-2765(01)00415-4)

Figure 3 Macromolecular Mimicry in Termination and Ribosome Recycling CPK representations of (left to right) human eRF1, E. coli RF2, yeast tRNAPhe, and Thermotoga maritima RRF. The orientation of eRF1 relative to tRNA is similar to what is proposed by Song et al. (2000), and the orientation of RRF relative to tRNA was proposed by Selmer et al. (1999). Molecular Cell 2001 8, 1375-1382DOI: (10.1016/S1097-2765(01)00415-4)