Mitochondrial content reflects oocyte variability and fertilization outcome  Teresa Almeida Santos, Ph.D., Shahy El Shourbagy, M.D., Justin C. St. John, Ph.D.  Fertility and Sterility  Volume 85, Issue 3, Pages 584-591 (March 2006) DOI: 10.1016/j.fertnstert.2005.09.017 Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

FIGURE 1 Real time PCR. Amplification of standards used to determine the number of mitochondria in a series of fertilized, unfertilized, and degenerate oocytes. Reaction conditions are described in the “Materials and Methods.” Legend: negative (blue); 1 in 10 dilution (pink); 1 in 100 dilution (yellow); 1 in 1,000 dilution (aqua); 1 in 10,000 dilution (purple); and 1 in 100,000 dilution (dark red). Santos. Mitochondria in oocytes and embryos. Fertil Steril 2006. Fertility and Sterility 2006 85, 584-591DOI: (10.1016/j.fertnstert.2005.09.017) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

FIGURE 2 Melt curve analysis. Melt curves were constructed following each reaction to determine whether mispriming had occurred in any reactions by performing Sybr Green fluorescence reactions at temperatures between 55–93°C. Any reactions perceived to contain mispriming were repeated to eliminate doubt. Santos. Mitochondria in oocytes and embryos. Fertil Steril 2006. Fertility and Sterility 2006 85, 584-591DOI: (10.1016/j.fertnstert.2005.09.017) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions