Rapid diagnosis of human brucellosis by SYBR Green I-based real-time PCR assay and melting curve analysis in serum samples M.I. Queipo-Ortuño, J.D. Colmenero,

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Presentation transcript:

Rapid diagnosis of human brucellosis by SYBR Green I-based real-time PCR assay and melting curve analysis in serum samples M.I. Queipo-Ortuño, J.D. Colmenero, J.M. Reguera, M.A. García-Ordoñez, M.E. Pachón, M. Gonzalez, P. Morata Clinical Microbiology and Infection Volume 11, Issue 9, Pages 713-718 (September 2005) DOI: 10.1111/j.1469-0691.2005.01202.x Copyright © 2005 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Examples of LightCycler (LC) printouts showing detection of Brucella DNA by the LC-PCR assay. (A) Fluorescent signal related to cycle number on a panel of ten-fold dilutions between 107 and 10 fg of Brucella abortus B-19 DNA. Brucella DNA samples were amplified in duplicate for 45 cycles. (B) Logarithmic standard curve of (A). Cp values are plotted against decreasing concentrations of Brucella DNA. The slope is −2.89 cycles/log10 and the correlation coefficient is 0.99. Clinical Microbiology and Infection 2005 11, 713-718DOI: (10.1111/j.1469-0691.2005.01202.x) Copyright © 2005 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 2 Examples of LightCycler (LC) printouts showing melting curves of the amplified fragments generated by the LC-PCR assay. Specific signals had melting temperatures of 88.16 ± 0.05°C. Clinical Microbiology and Infection 2005 11, 713-718DOI: (10.1111/j.1469-0691.2005.01202.x) Copyright © 2005 European Society of Clinical Infectious Diseases Terms and Conditions