FLT Given a photosynthesis role play I will be able to model the steps of the light reaction and calvin cycle through manipulates and partner

Light Reaction

Everyone will take part in our photosynthesis theather Part 1--Actors 1) Photosystem II 2) ETC 3) Last ETC 4) Photosystem I 5) ATP synthase 6) Sun 7) Stroma 8) Water 9) ADP (also in part III) 10) NADP+(also in part III) 11) Thylakoid space

Roles for Part I Sun: Holds flashlight and shines it on Photosystems PS II: passes e- cards to last ETC ETC: Passes e- cards along line and pumps H+ cards over shoulder to thylakoid space PS I: Passes e- cards to LAST ETC WATER: Passes e- cards to PS II THYLAKOID SPACE:  Receives H+ cards from ETC and passes H+ cards to ATP synthase ATP SYNTHASE:  receives H+ cards from thylakoid space  and passes them to stroma; holds P cards and attaches them to ADP. LAST ETC: collects all e- cards as they come down line, receives H+ from stroma and gives 2 e- cards and attaches an H+ to NADP+ ACT OUT ETC first. Where is this happening? What are the jobs? Where do electrons come from to replace those lost from PS II? Where do ATP and NADPH go next?

Arrange yourselves Students wear appropriate name tags and line up in correct position. Photosystem II, ETC, ETC, ETC, Photosystem I, ETC, ATP synthase stand in a line Sun stands to side of PS II. Stroma stands in front of line. Water stands next to thylakoid space. ADP stands near ATP synthase NADP+ stands near ATP synthase and last ETC

Light Reaction

Actors—Calvin Cycle 5 Actors: 1 CO2 ATP and NADPH from light reactions 1 Stroma 1 RuBP Stroma, “Hi, I’m stroma. The Calvin Cycle happens around me” CO2 enters and high fives stroma, “Hi, I’m CO2” (circle stroma) RuBP enters and high fives stroma “Hi, I’m RuBP” (circle stroma CO2 and RuBP link arms . Both say “We are joined by RUBISCO. We are now a 6 carbon molecule. Too bad we are unstable. Let’s become two 3 carbon molecules. Just call us PGA.” ATP removes its P and says “I am now ADP and are low energy. Back to the light reaction.” NADPH removes its H+ and passes it and 2 e- cards to STROMA.  “I am now NADP+ and are missing electrons. Back to the light reaction.” STROMA takes 2 e- and H+ from NADPH, and energy and P from ATP. STROMA yells ”I need 2 more CO2 and RuBP so I can make G3P and recycle RuBP”

Part II SPLITTING WATER: Assign 8 students to be water molecules in THYLAKOID SPACE. Each starts wearing an O card with 2 H's attached and holds 2 e- cards. As water is split, the H+ cards are detached and are passed to the THYLAKOID SPACE. The 2 e- cards pass to PS II, and the leftover O holds hands and joins with another O to become O2 and leaves the chloroplast (room).

Calvin Cycle

Calvin cycle 1C 5C 6C 5C 3C 3C 3C CO2 RuBP RuBisCo 3 ATP 3 ADP used 1. Carbon fixation C 3. Regeneration of RuBP C 5C C RuBP RuBisCo C ribulose bisphosphate 6C starch, sucrose, cellulose & more 3 ADP 3 ATP C ribulose bisphosphate carboxylase 5C C used to make glucose C glyceraldehyde-3-P C 3C PGA 1. A five-carbon sugar molecule called ribulose bisphosphate, or RuBP, is the acceptor that binds CO2 dissolved in the stroma. This process, called CO2 fixation, is catalyzed by the enzyme RuBP carboxylase, forming an unstable six-carbon molecule. This molecule quickly breaks down to give two molecules of the three-carbon 3-phosphoglycerate (3PG), also called phosphoglyceric acid (PGA). 2. The two 3PG molecules are converted into glyceraldehyde 3-phosphate (G3P, a.k.a. phosphoglyceraldehyde, PGAL) molecules, a three-carbon sugar phosphate, by adding a high-energy phosphate group from ATP, then breaking the phosphate bond and adding hydrogen from NADH + H+. 3. Three turns of the cycle, using three molecules of CO2, produces six molecules of G3P. However, only one of the six molecules exits the cycle as an output, while the remaining five enter a complex process that regenerates more RuBP to continue the cycle. Two molecules of G3P, produced by a total of six turns of the cycle, combine to form one molecule of glucose. G3P C phosphoglycerate C C C 3C C = C C C 6 ADP 6 ATP 2. Reduction C | H C – C 6 NADP 6 NADPH 3C C 13

Calvin cycle when CO2 is abundant RuBP ADP ATP RuBisCo 6C unstable intermediate G3P to make glucose G3P 5C C3 plants 3C PGA 3C NADP NADPH ADP ATP 3C 14

Sun: Holds flashlight and shines it on Photosystems

PS II: passes e- cards to last ETC

ETC: Passes e- cards along line and pumps H+ cards over shoulder to thylakoid space

PS I: Passes e- cards to LAST ETC

WATER: Passes e- cards to PS II

THYLAKOID SPACE:  Receives H+ cards from ETC and passes H+ cards to ATP synthase

ATP SYNTHASE:  receives H+ cards from thylakoid space  and passes them to stroma; holds P cards and attaches them to ADP.

LAST ETC: collects all e- cards as they come down line, receives H+ from stroma and gives 2 e- cards and attaches an H+ to NADP+

e-

ATP SYNTHASE

ADENOSINE P P

ADENOSINE P P

ADENOSINE P P

ADENOSINE P P

ADENOSINE P P

P P P P P P

ETC

ETC

ETC

ETC

CO2 CO2 CO2 CO2 CO2 CO2

CO2 CO2 CO2 CO2 CO2 CO2

CO2 CO2 CO2 CO2 CO2 CO2

WATER (H2O)

NADP +

NADP +

NADP +

NADP +

NADP +

H+ H+ H+ H+ H+ H+

H+ H+ H+ H+ H+ H+

H+ H+ H+ H+ H+ H+

O

PHOTOSYSTEM I

PHOTOSYSTEM II

CALVIN CYCLE

SUN

GLUCOSE C C C C C C

STROMA

THYLAKOID SPACE

Rubisco

P-C-C-C-C-C-P RuBP

P-C-C-C-C-C-P RuBP

P-C-C-C-C-C-P RuBP

C-C-C-P

C-C-C-P

C-C-C-P

C-C-C-P

C-C-C-P

C-C-C-P

Narrated Photosynthesis http://vcell.ndsu.edu/animations/photosynthesis/movie-flash.htm

Cellular respiration and photosynthesis quiz http://w3.dwm.ks.edu.tw/bio/activelearner/08/ch8c1.html

Make a comic strip explaining photosynthesis Characters Chloroplast (stroma + thylakoid) ATP Stroma CO2 Sunlight H2) NADP+ NADPH Membrane Proteins H+ ATP synthase Glucose RuBP Rubisco Electrons O2