CHMI 2227E Biochemistry I Enzymes: Regulation

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Presentation transcript:

CHMI 2227E Biochemistry I Enzymes: Regulation CHMI 2227 - E.R. Gauthier, Ph.D.

Regulation of enzyme activity CHMI 2227 - E.R. Gauthier, Ph.D.

Regulation of enzyme activity 1. Allostery CHMI 2227 - E.R. Gauthier, Ph.D.

Regulation of enzyme activity 1. Allostery ATCase Asp Carbomoyl phosphate CHMI 2227 - E.R. Gauthier, Ph.D.

Regulation of enzyme activity 1. Allostery - ATCase CHMI 2227 - E.R. Gauthier, Ph.D.

Regulation of enzyme activity 1. Allostery - ATCase CTP ATCase CHMI 2227 - E.R. Gauthier, Ph.D.

Regulation of enzyme activity 2. Regulatory subunits ATP cAMP Adenylate Cyclase Phosphodiesterase AMP Caffeine CHMI 2227 - E.R. Gauthier, Ph.D.

Regulation of enzyme activity 3. Regulation by covalent modification CHMI 2227 - E.R. Gauthier, Ph.D.

Regulation of enzyme activity 3. Regulation by phosphorylation CHMI 2227 - E.R. Gauthier, Ph.D.

Regulation of enzyme activity 3. Phosphorylation  Adrenaline b g G Protein Adrenaline receptor Adenylate cyclase ATP cAMP Protein Kinase A (inactive) ↑Intracellular glucose PKA-cAMP (active) ↑Energy Run Like Hell! Glycogen (glucose stores) Phosphorylase kinase Kinase-PO4 Phosphorylase-PO4 Inside the cell Outside the cell CHMI 2227 - E.R. Gauthier, Ph.D.

Regulation of enzyme activity 4. Regulation of enzyme stability CHMI 2227 - E.R. Gauthier, Ph.D.

Regulation of enzyme activity 4. Regulation of enzyme stability Ub E1 E2 E3 CHMI 2227 - E.R. Gauthier, Ph.D.

Regulation of enzyme activity 4. Regulation of enzyme stability Ub Degraded enzyme Proteasome CHMI 2227 - E.R. Gauthier, Ph.D.

Regulation of enzyme activity 5. Limited proteolysis CHMI 2227 - E.R. Gauthier, Ph.D.

Regulation of enzyme activity 5. Limited proteolysis CHMI 2227 - E.R. Gauthier, Ph.D.

Regulation of enzyme activity 5. Limited proteolysis Small intestine Secreted by pancreas CHMI 2227 - E.R. Gauthier, Ph.D.

Regulation of enzyme activity 5. Limited proteolysis CHMI 2227 - E.R. Gauthier, Ph.D.