Identification of the host interaction partners of HEV proteins in the human liver and fetal brain cDNA libraries. Identification of the host interaction.

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Identification of the host interaction partners of HEV proteins in the human liver and fetal brain cDNA libraries. Identification of the host interaction partners of HEV proteins in the human liver and fetal brain cDNA libraries. (A) Schematic of the g-1 HEV genome and of the proteins encoded by it. UTR, untranscribed region. (B) (Upper panel) Western blot showing GAPDH protein level in Huh7 cell extract expressing the indicated Flag-tagged viral proteins. (Lower left panel) Coimmunoprecipitation of Flag-tagged viral proteins expressed in Huh7 cells using Flag-agarose beads and Western blotting using anti-Flag antibody. The right panel shows a higher exposure of the same blot (cropped) to reveal the signal of Met-Flag protein. The crosshatch symbol (#) denotes that PCP and Hel and V and ORF4 migrate at similar sizes. Mol Wt, molecular weight. (C) (Upper panel) Western blot showing GAPDH protein level in Huh7 cell extract expressing the indicated HA-tagged viral proteins. (Lower panel) Coimmunoprecipitation of HA-tagged viral proteins expressed in Huh7 cells using HA-agarose beads and Western blotting using anti-HA antibody. (D) Aliquots of CoIP samples (shown in panels B and C) were immunoblotted using the indicated antibodies. Chandru Subramani et al. mSystems 2018; doi:10.1128/mSystems.00135-17