Ilse Rooman, Jessy Lardon, Daisy Flamez, Frans Schuit, Luc Bouwens

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Mitogenic effect of gastrin and expression of gastrin receptors in duct-like cells of rat pancreas Ilse Rooman, Jessy Lardon, Daisy Flamez, Frans Schuit, Luc Bouwens Gastroenterology Volume 121, Issue 4, Pages 940-949 (October 2001) DOI: 10.1053/gast.2001.27998 Copyright © 2001 American Gastroenterological Association Terms and Conditions

Fig. 1 Fold increase in BrdU-labeling index of monolayers of transdifferentiated cells compared with control values after exposure to cerulein (Cae) at 10 and 100 nmol/L and to tetragastrin (TG), pentagastrin (PG), gastrin I (gasI), and sulphated gastrin II (gasII) at 0.1, 1, and 10 nmol/L. *P < 0.05. Gastroenterology 2001 121, 940-949DOI: (10.1053/gast.2001.27998) Copyright © 2001 American Gastroenterological Association Terms and Conditions

Fig. 2 Western blot of total extract from normal adult rat pancreas (Pan) and from isolated acinar cell preparations after 1, 2, 3, and 4 days of culture with a CCK-B receptor–specific antiserum. Gastroenterology 2001 121, 940-949DOI: (10.1053/gast.2001.27998) Copyright © 2001 American Gastroenterological Association Terms and Conditions

Fig. 3 (A) Immunocytochemistry for CCK-B receptor on monolayers of duct-like cells, and paraffin sections (C) of the pancreatic tail 5 days after duct ligation, (E) of fetal pancreas E17, and (F) of normal pancreas. Immunoreactivity was completely abolished after preabsorption of the antibody with the corresponding antigen in (B) cultures of duct-like cells and (D) in vivo in the ductal complexes. Gastroenterology 2001 121, 940-949DOI: (10.1053/gast.2001.27998) Copyright © 2001 American Gastroenterological Association Terms and Conditions

Fig. 4 RT-PCR analysis for CCK-B receptor mRNA and actin mRNA in RNA extracts from (lane A) adult rat pancreatic unligated head, (lane B) the ligated tail part, and (lane C) purified rat endocrine non-β cells. Gastroenterology 2001 121, 940-949DOI: (10.1053/gast.2001.27998) Copyright © 2001 American Gastroenterological Association Terms and Conditions

Fig. 5 Immunocytochemical staining for (A,C,E) amylase and (B,D,F) the duct cell marker CK20. (A and B) Starting preparations and (C–F) samples of cells (C and D) after 4 days in control cultures and (E and F) after 4 days in culture conditions with L-365,260 (100 nmol/L); bar = 30 μm. Gastroenterology 2001 121, 940-949DOI: (10.1053/gast.2001.27998) Copyright © 2001 American Gastroenterological Association Terms and Conditions