Heat shock protein (HSP 72) expression in patients undergoing cardiac operations Lynn B. McGrath, MD, Marius Locke, PhD, Michael Cane, MD, Chao Chen,

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Presentation transcript:

Heat shock protein (HSP 72) expression in patients undergoing cardiac operations Lynn B. McGrath, MD, Marius Locke, PhD, Michael Cane, MD, Chao Chen, PhD, C.David Ianuzzo, PhD The Journal of Thoracic and Cardiovascular Surgery Volume 109, Issue 2, Pages 370-376 (February 1995) DOI: 10.1016/S0022-5223(95)70399-3 Copyright © 1995 Mosby, Inc. Terms and Conditions

Fig. 1 Fluorographic analyses of IEF/SDS-PAGE separated proteins from human lymphocytes labeled with [35S]-methionine at either 37 degrees C (control, A) or 43 degrees C (heat shock, B). Stress proteins (HSP 72, HSC 73, and HSP 90) are indicated by downward arrows. Actin (A) and a 70 kDa nonshock stress protein (upward arrow) whose synthesis decreased after heat shock are also included. Gels were processed as outlined in Methods. SDS, Sodium dodecyl sulfate; IEF, isoelectric focus. The Journal of Thoracic and Cardiovascular Surgery 1995 109, 370-376DOI: (10.1016/S0022-5223(95)70399-3) Copyright © 1995 Mosby, Inc. Terms and Conditions

Fig. 2 SDS-PAGE and HSP 72 analyses from the heart of a patient with coronary artery disease. A, The myocardial proteins separated by SDS-PAGE and stained with Coomassie blue. B, A gel identical to that in A, but blotted and reacted with a monoclonal antibody for HSP 72. Both panels: lane 1, before bypass; lane 2, after reperfusion; lane 3, after bypass. MW, Molecular weight; Stds, standards. The Journal of Thoracic and Cardiovascular Surgery 1995 109, 370-376DOI: (10.1016/S0022-5223(95)70399-3) Copyright © 1995 Mosby, Inc. Terms and Conditions

Fig. 3 Graphic representation of HSP 72 content (A), glycogen (B), and lactate (C) concentrations before bypass, after reperfusion, and after bypass. The Journal of Thoracic and Cardiovascular Surgery 1995 109, 370-376DOI: (10.1016/S0022-5223(95)70399-3) Copyright © 1995 Mosby, Inc. Terms and Conditions

Fig. 3 Graphic representation of HSP 72 content (A), glycogen (B), and lactate (C) concentrations before bypass, after reperfusion, and after bypass. The Journal of Thoracic and Cardiovascular Surgery 1995 109, 370-376DOI: (10.1016/S0022-5223(95)70399-3) Copyright © 1995 Mosby, Inc. Terms and Conditions

Fig. 3 Graphic representation of HSP 72 content (A), glycogen (B), and lactate (C) concentrations before bypass, after reperfusion, and after bypass. The Journal of Thoracic and Cardiovascular Surgery 1995 109, 370-376DOI: (10.1016/S0022-5223(95)70399-3) Copyright © 1995 Mosby, Inc. Terms and Conditions