Direct Detection of Singlet Oxygen Generated by UVA Irradiation in Human Cells and Skin  Jürgen Baier, Tim Maisch, Max Maier, Michael Landthaler, Wolfgang Bäumler  Journal of Investigative Dermatology  Volume 127, Issue 6, Pages 1498-1506 (June 2007) DOI: 10.1038/sj.jid.5700741 Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Singlet oxygen detection in pig skin ex vivo. (a) The time-resolved singlet oxygen luminescence at 1,270nm. (b) The luminescence signal at different wavelengths in the range from 1,150 to 1,400nm. Journal of Investigative Dermatology 2007 127, 1498-1506DOI: (10.1038/sj.jid.5700741) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Singlet oxygen detection in the human skin in vivo. (a) The time-resolved singlet oxygen luminescence at 1,270nm. (b) The luminescence signal shown at different wavelengths in the range from 1,150 to 1,400nm. Journal of Investigative Dermatology 2007 127, 1498-1506DOI: (10.1038/sj.jid.5700741) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Singlet oxygen detection in human primary keratinocytes (NHEK). The time-resolved singlet oxygen luminescence (a) at 1,270nm and (b) with sodium azide. (c) The luminescence signal shown at different wavelengths in the range from 1150 to 1400nm of NHEK without sodium azide. Journal of Investigative Dermatology 2007 127, 1498-1506DOI: (10.1038/sj.jid.5700741) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Singlet oxygen detection in phosphatidylcholine dissolved in ethanol (15mg/ml). (a) The time-resolved singlet oxygen luminescence at 1,270nm. (b) The luminescence signal shown at different wavelengths in the range from 1,150 to 1,400nm. Journal of Investigative Dermatology 2007 127, 1498-1506DOI: (10.1038/sj.jid.5700741) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Singlet oxygen detection in phosphatidylcholine dissolved in water (15mg/ml). (a) The time-resolved singlet oxygen luminescence at 1,270nm. (b) The luminescence signal shown at different wavelengths in the range from 1,150 to 1,400nm. Journal of Investigative Dermatology 2007 127, 1498-1506DOI: (10.1038/sj.jid.5700741) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 6 Upregulation of IL-12 in NHEK 16hours after exposure to UVA light at 355nm. The fluence ranged from 0 to 16J/cm2, IL-12 expression increased with increasing fluence. The dashed line indicates the threshold of IL-12 detection (10pg/ml). Journal of Investigative Dermatology 2007 127, 1498-1506DOI: (10.1038/sj.jid.5700741) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions