Searching the NCBI Databases

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Searching the NCBI Databases

Search for CFTR[gene] in the Protein database What is it?

Limit to genes in human only CFTR[gene] AND human[orgn] How many?

Restrict to RefSeq How many?

RefSeq records can also be filtered using the Limits tab

Examine the NP_000483 record How many amino-acids are in the protein? On which chromosome is this protein-coding gene located?

Follow the dbSNP link to examine the SNPs associated with this protein How many SNPs?

Follow the dbSNP link to examine the SNPs associated with this protein Describe mutation in first record. Is it synonymous?

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Sequence Retrieval

Search for human BRCA1 in Protein database Select longest isoform

Display the sequences in FASTA format

Save the sequence into a WORD file