Overexpression of Laminin-8 in Human Dermal Microvascular Endothelial Cells Promotes Angiogenesis-Related Functions Jie Li, Lisa Zhou, Hoang T. Tran,

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Overexpression of Laminin-8 in Human Dermal Microvascular Endothelial Cells Promotes Angiogenesis-Related Functions Jie Li, Lisa Zhou, Hoang T. Tran, Yi Chen, Ngon E. Nguyen, Marvin A. Karasek, M. Peter Marinkovich Journal of Investigative Dermatology Volume 126, Issue 2, Pages 432-440 (February 2006) DOI: 10.1038/sj.jid.5700089 Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 (a) Laminin expression in primary HDMECs. Anti-laminin α5 mAb 4C7 (lane 1), anti-laminin γ1 mAb 2E8 (lane 2), anti-laminin β1 mAb 545 (lane 3), anti-laminin α2 chain antibody 5H2 (lane 4), or mouse IgG (lane 5) were used to immunoprecipitate laminins from HDMEC medium. Precipitated proteins were washed extensively, then dissolved in sample buffer and separated by SDS-PAGE under non-reducing conditions on a 3–5% acrylamide gel, and then visualized by autoradiography. (b) Western blot analysis of HDMEC laminins. Conditioned medium of LSV5 keratinocytes (lanes 1, 4, 6, and 8) or HDMECs (lanes 2, 5, 7, and 9) or HDMEC matrix (lane 3) was separated by non-reduced SDS-PAGE, transferred to nitrocellulose, and analyzed with the following primary antibodies: human placental laminin pAb (lanes 1–3), laminin-5/6 pAb (lanes 4 and 5), laminin α4 pAb (lanes 6 and 7), non-immune rabbit sera (lanes 8 and 9). (c) Reduced SDS-PAGE analysis of HDMEC laminins. Conditioned medium of HDMECs was separated on 5% acrylamide gels by reduced SDS-PAGE and analyzed by Western blot with the following antibodies: human placental laminin pAb (lane 1), Engelbaeth-Holm-Swarm sarcoma laminin pAb (lane 2), laminin α4 pAb (lane 3), and non-immune rabbit sera (lane 4). (d) Non-reduced Western blot of conditioned HDMEC-LACZ (control) or HDMEC-α4 (α4) medium using human placental laminin pAb. Positions of laminin-10 and -8 are shown to the right. Journal of Investigative Dermatology 2006 126, 432-440DOI: (10.1038/sj.jid.5700089) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Laminin-8 promotes epithelial morphology of HDMECs. HDMECs were transduced (b, d, f, and h) with full-length laminin α4 chain sense cDNA or (a, c, e, and g) with retroviral vector alone as control and selected with Blasticidin. (a) Phase-contrast photography revealed typical mixture of epithelioid and spindled cells. (b) Laminin α4 sense-transduced cells were of epithelioid morphology. Both cell types were examined by immunohistochemistry using antibodies directed to (c and d) von Willebrand factor, (e and f) PECAM, and (g and h) type IV collagen. Bar=20μm. Journal of Investigative Dermatology 2006 126, 432-440DOI: (10.1038/sj.jid.5700089) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 (a–d) Laminin-8 promotes HDMEC tubule formation. (a, c) HDMECs-α4, (b, d) HDMEC-LACZ were evaluated by tubule/vessel formation collagen overlay assay and observed by phase-contrast microscopy at (a, b) 4hours and (c, d) 24hours. (e–i) HDMEC tubule formation via α2β1 and α6β1 integrins. HDMECs-α4 were grown to confluence, and incubated with integrin antibodies overnight prior to collagen gel overlay and observed at 4, 8, and 24hours. Antibody of (f) α2 integrin or (g) α6 integrin alone could partially block tubule formation at 8hours, (i) β1 integrin alone or (h) α2 and α6 integrins together could completely block the tubule formation at 8hours. Panel e is control with mouse IgG. Bar=200μm. Journal of Investigative Dermatology 2006 126, 432-440DOI: (10.1038/sj.jid.5700089) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 (a–d) Laminin-8 promotes HDMEC migration. (b, d) HDMEC-α4 or (a, c) HDMEC-LACZ were grown to confluence in 35mm dishes. Cross-shaped scratches were made and cells were analyzed at (a, b) 0 and (c, d) 24hours. (e–j) Antibody inhibition of HDMEC migration. HDMEC-α4 cells were evaluated by scratch assay at (e) 0 or (f–j) 24hours in the presence of inhibitory antibodies to integrins (g) α6, (h) αvβ3, (i) β1, (j) β1 and αvβ3 together or (b) mouse IgG. Bar=200μm. Journal of Investigative Dermatology 2006 126, 432-440DOI: (10.1038/sj.jid.5700089) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Functional analysis of recombinant laminin α4G domain. (a) Conditioned medium of laminin α4G domain, expressing 293-EBNA cells (lanes 1 and 3) or control parental 293-EBNA cells (lanes 2 and 4) was purified by FLAG antibody affinity chromatography, separated by SDS-PAGE, and stained with Coomassie Blue total protein stain (lanes 1 and 2) or with anti-laminin α4 pAb (lanes 3 and 4). (b) Improved spreading and flattening of HDMECs on G1–3 coated culture surfaces. (c) The attachment of endothelial cells to laminin α4G domain via β1 and αvβ3 integrins. The 96-well plates were pre-coated with 10μg/ml laminin α4G1-3 domain protein or BSA as control, and then utilized in HDMEC colorimetric cell attachment assays in the presence of 15μg/ml of each of the various integrin-blocking antibodies as indicated under the bars. The attached cells were stained with 0.1% crystal violet and analyzed by colorimetric measurement at OD570. (d) Laminin α4G domain promotes HDMEC migration via αvβ3 integrin. The bottoms of Costar migration chamber were pre-coated with BSA (left bar) or laminin α4 G1-3 protein (all other condition shown on the right), HDMECs were then plated on the top of the migration chambers and immerged in the growth medium containing mouse IgG 15μg/ml (control) or the indicated antibodies at the same concentration, and cells were counted in the lower chamber after 24hours. Journal of Investigative Dermatology 2006 126, 432-440DOI: (10.1038/sj.jid.5700089) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions