Salmonella genomic island 1-J variants associated with change in the antibiotic resistance gene cluster in multidrug-resistant Salmonella enterica serovar.

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Salmonella genomic island 1-J variants associated with change in the antibiotic resistance gene cluster in multidrug-resistant Salmonella enterica serovar Virchow isolated from humans, Taiwan, 2004–2006  C. Chu, B. Doublet, Y.-L. Lee, A. Cloeckaert, C.-S. Chiou, S.-W. Chen, C.-W. Lin, C.-H. Chiu  Clinical Microbiology and Infection  Volume 18, Issue 1, Pages 47-53 (January 2012) DOI: 10.1111/j.1469-0691.2011.03464.x Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Dendrograms constructed with XbaI-PFGE patterns for 98 S. Virchow isolates. SGI1 variants are listed in Table S2., resistant; █, sensitive. □ for ampicillin, C for chloramphenicol, S for streptomycin, Sxt for sulfamethoxazole/trimethoprim, and T for tetracycline. The dendrogram was generated by the the unweighted pair group method with arithmetic mean (UPGMA), using the Dice-predicted similarity value of two patterns. The statistics program was provided by BIONUMERICS version 4.5, with settings of 1.0% optimization and 0.7% tolerance. Isolates B94 and B98 were isolated in 1994. H9812 is S. Braenderup used as standard size marker for PFGE analysis. Clinical Microbiology and Infection 2012 18, 47-53DOI: (10.1111/j.1469-0691.2011.03464.x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Genetic organization of the complex class 1 integrons in the different SGI1-J variants from S. Virchow strains. Black and grey arrows correspond to SGI1 antibiotic resistance genes and chromosomal genes flanking SGI1, respectively. DR-L and DR-R are the 18-bp left and right direct repeats, respectively, bracketing SGI1. IRi and IRt are 25 bp imperfect inverted repeats defining the left and right end of complex class 1 integrons. The usual insertion point of the complex integron In104 or variants of it in SGI1 and the 5 bp target site duplication flanking the complex integron (InSGI1-J3, -J4) in ORF S023 are indicated (GenBank accession number EU924797). The p1-9 probe and XbaI restriction sites (X) are indicated. Clinical Microbiology and Infection 2012 18, 47-53DOI: (10.1111/j.1469-0691.2011.03464.x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions