Reduced cell motility and expression of phosphorylated FAK in a fibroblast cell line of an individual with T3257I mutation. Reduced cell motility and expression.

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Reduced cell motility and expression of phosphorylated FAK in a fibroblast cell line of an individual with T3257I mutation. Reduced cell motility and expression of phosphorylated FAK in a fibroblast cell line of an individual with T3257I mutation. (A) Representative images of WT and mutant dermal fibroblast wound healing assays in the presence of PDGF versus vehicle alone. Note the attenuation of motility in TNXB mutant-expressing fibroblasts (Mut T3257I) in response to PDGF relative to the WT cell line. (B) The results of three individual wound healing assays expressed as percent wound healing are quantified. (C) Representative images of immunoblots from WT and mutant fibroblasts with staining for TNXB, FAK, and pY397-FAK. Note that TNXB expression is similar in all groups and that the PDGF-induced phosphorylation of FAK at tyrosine 397 is significantly attenuated in mutant (Mut T3257I) versus the WT fibroblast cell line. Rasheed A. Gbadegesin et al. JASN 2013;24:1313-1322 ©2013 by American Society of Nephrology