Linda M. de Heer, MD, Ricardo P. J. Budde, MD, PhD, Evert-Jan P. A

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Computed Tomography Detects Tissue Formation in a Stented Engineered Heart Valve  Linda M. de Heer, MD, Ricardo P.J. Budde, MD, PhD, Evert-Jan P.A. Vonken, MD, PhD, Frank P.T. Baaijens, PhD, Paul F. Gründeman, MD, PhD, Lex A. van Herwerden, MD, PhD, Simon P. Hoerstrup, MD, PhD, Jolanda Kluin, MD, PhD  The Annals of Thoracic Surgery  Volume 92, Issue 1, Pages 344-345 (July 2011) DOI: 10.1016/j.athoracsur.2010.12.063 Copyright © 2011 The Society of Thoracic Surgeons Terms and Conditions

Fig 1 Computed tomographic (256-slice) images of the stented construct. (A) Long axis of the tissue-engineered heart valves with the nitinol stent. The black inner lining of the stent is the tissue-engineered wall of the construct. The tissue-engineered leaflets are indicated by white asterisks. (B) The short axis images at two distinct levels are shown (red line, distal of the valve leaflets) and (C) (blue line, through the valve leaflets). (B) There is a lack of in-stent tissue formation above the level of the leaflets (absence of the black inner lining delineated by the yellow dotted lines). (C) Similarly, at the level of the valve leaflets (the leaflets are indicated by white asterisks) about one fifth of the circumference is missing (absence of the black inner lining delineated by the yellow dotted lines). Note the absence of metal artefacts by the nitinol stent. The Annals of Thoracic Surgery 2011 92, 344-345DOI: (10.1016/j.athoracsur.2010.12.063) Copyright © 2011 The Society of Thoracic Surgeons Terms and Conditions

Fig 2 (A) Three-dimensional volume-rendered reconstruction of the valved stent from above. The yellow ellipsis indicates the small area without tissue formation. (B, C) The macroscopic photographs demonstrate excellent correlation between the explant and the computed tomographic image. The asterisks indicate the top of the valve leaflets. The Annals of Thoracic Surgery 2011 92, 344-345DOI: (10.1016/j.athoracsur.2010.12.063) Copyright © 2011 The Society of Thoracic Surgeons Terms and Conditions