NS3 inhibits endogenous miRNA action.

Slides:

Advertisements

Similar presentations

OTUB1 increases RAS activity and enhances MAPK activation in wt RAS cells OTUB1 increases RAS activity and enhances MAPK activation in wt RAS cells A,

Advertisements

Selective inhibition of BCL10-induced NF-κB activation by Tat–Peptide VIII HEK-293 cells were transiently co-transfected with an expression vector encoding.

Prolyl‐hydroxylase‐domain proteins regulate hypoxia inducible factor‐α in response to O2 availability. Prolyl‐hydroxylase‐domain proteins regulate hypoxia.

Induction of mPer1 mRNA expression by prostaglandin E2 (PGE2) in NIH3T3 cells. Induction of mPer1 mRNA expression by prostaglandin E2 (PGE2) in NIH3T3.

Myc‐induced miRNAs attenuate differentiation of c‐Myc‐depleted ES cells on LIF withdrawal. Myc‐induced miRNAs attenuate differentiation of c‐Myc‐depleted.

Knockdown of GW182 and TNRC6B impaired miRNA stability.

SncRNA715 levels are increased in chronic MS lesions.

Colocalization of the human trimethylguanosine synthase 1 and survival of motor neuron proteins. Colocalization of the human trimethylguanosine synthase.

NS3 has no interferon and PKR antagonistic properties.

IRES‐dependent translation is specifically abolished by an IRES point mutation. IRES‐dependent translation is specifically abolished by an IRES point mutation.

Resveratrol inhibits the transactivation potential of the RelA/p65 protein. Resveratrol inhibits the transactivation potential of the RelA/p65 protein.

Expression of adenylyl cyclases and miR‐142‐3p in CD4+CD25− T cells and CD4+CD25+ regulatory T cells. Expression of adenylyl cyclases and miR‐142‐3p in.

Nur77 interacts with COUP‐TF in yeast.

SncRNA715 expression in oligodendrocytes.

Telomerase activity in smooth‐muscle cells increases lifespan and extends telomeres. Telomerase activity in smooth‐muscle cells increases lifespan and.

Yeast Tgl3p expressed in HeLa cells localizes to lipid droplets.

JNK is activated by Wnt5a.

Phosphorylation of RelA Ser311 in vivo.

Murine Nod1 preferentially detects diaminopimelic acid‐type muramyl tetrapeptides. Murine Nod1 preferentially detects diaminopimelic acid‐type muramyl.

The N‐terminus and SH2 domain of SOCS‐1 contribute to inhibition of JAK1 and JAK2 kinase activity. The N‐terminus and SH2 domain of SOCS‐1 contribute to.

Cytochrome c and ATP hydrolysis are required for Apaf‐1 to interact with procaspase‐9. Cytochrome c and ATP hydrolysis are required for Apaf‐1 to interact.

TCR‐mediated IL‐2 promoter activation in J.Vav1 cells.

Double‐stranded RNA binding of NS3 is required for HIV‐1 Tat complementation. Double‐stranded RNA binding of NS3 is required for HIV‐1 Tat complementation.

AO domain of LSD1 is responsible for its interaction with Snail1.

Optical labelling by two‐photon excitation.

GLUT3 is a direct target of TCF4/β‐catenin

The 5′‐terminal structure of the miRNA gene cluster miR‐23a∼27a∼24‐2.

SncRNA715 regulates MBP translation.

Relocalisation of both MK5 and ERK3 requires a CRM1‐dependent nuclear export pathway. Relocalisation of both MK5 and ERK3 requires a CRM1‐dependent nuclear.

Functional properties of pRb.

Non‐specific activation of gene transcription by exogenous Fe65 in luciferase‐based reporter assays. Non‐specific activation of gene transcription by exogenous.

Nore1 enhances interaction between HIPK1 and Mdm2.

Ubiquitination of Drp1 by MARCH‐V.

Inhibition of RAS‐dependent NIH 3T3 cell transformation activity by anti‐RAS scFv. Inhibition of RAS‐dependent NIH 3T3 cell transformation activity by.

Expression levels of the transgenic DIA1(R1204X) mutant in the inner ear of TG mice Expression levels of the transgenic DIA1(R1204X) mutant in the inner.

Mutations affecting HOIP–UBA and HOIL‐1L–UBL interaction that compromise NF‐κB activation. Mutations affecting HOIP–UBA and HOIL‐1L–UBL interaction that.

MCPH1 interacts with E2F1. MCPH1 interacts with E2F1. (A,B) HEK293 cells were left untreated (No tx) or treated with 300 ng/ml NCS for 4 h or 2 μM adriamycin.

Reactive oxygen species inhibit miR‐199a and miR‐125b expression levels in vitro and in vivo. Reactive oxygen species inhibit miR‐199a and miR‐125b expression.

MiR‐199a directly targets ERBB2 and ERBB3, whereas miR‐125b targets ERBB3. miR‐199a directly targets ERBB2 and ERBB3, whereas miR‐125b targets ERBB3. (A)

Functional miR‐33 responsive elements in the 3′UTR of ATP8B1 and ABCB11.A,B.Conserved sequences in the 3′UTR of ATP8B1 and ABCB11 are partially complementary.

Crb reduces γ‐Secretase activity.

The MSP domain of MOSPD2 is sufficient to interact with STARD3 and STARD3NL The MSP domain of MOSPD2 is sufficient to interact with STARD3 and STARD3NL.

DDX3 is required for TBK1/IKKε‐dependent promoters and is recruited to IKKε. DDX3 is required for TBK1/IKKε‐dependent promoters and is recruited to IKKε.

Expression of PD‐1 on PBMC and malignant exudate T cells

Identification of miR‐499 targets

Rab22A localizes to the E/SE and REs and regulates RE dynamics

The de‐ubiquitinase OTUB1 interacts with the RAS GTPases

WDR11 forms a tertiary complex with EMX1 and GLI3

Zc3h10 downregulation impairs mitochondrial function and C2C12 differentiation Zc3h10 downregulation impairs mitochondrial function and C2C12 differentiation.

Lack of GTSF1 causes defects in MILI‐directed piRNA amplification

Activated Gα13 inhibits AhR‐mediated transcriptional activity.

The ERK pathway mediates the effect of EGF on LIMT, which normally inhibits mammary cell migration and invasion The ERK pathway mediates the effect of.

RIG‐I (N) and LRRC25 interact with ISG15 in an unconjugated manner, related to Figs 6 and 7 RIG‐I (N) and LRRC25 interact with ISG15 in an unconjugated.

IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice ARepresentative.

Improvement of shHBV7 specificity and efficiency by co‐expression of a TuD against the sense strand Improvement of shHBV7 specificity and efficiency by.

NDP52 promotes CHIKV infection in a species‐specific manner.

High‐throughput screen to identify a new regulator of mitochondrial function High‐throughput screen to identify a new regulator of mitochondrial function.

Induction of LIMD1 in hypoxia inhibits HIF‐1‐mediated gene expression

Knock‐in mutation of the T1348N mutation that ablates GTP binding to LRRK2 abolishes Rab10 and biomarker phosphorylation Knock‐in mutation of the T1348N.

Scheme of FGF‐dependent regulation of TGFβ signaling in smooth muscle cells and endothelial cells Scheme of FGF‐dependent regulation of TGFβ signaling.

Protein tyrosine kinase 7 is involved in Wnt/β‐catenin canonical signalling. Protein tyrosine kinase 7 is involved in Wnt/β‐catenin canonical signalling.

USP2a-dependent miRs deregulation modulates MYC expression.

Negative Regulation of Tumor Suppressor p53 by MicroRNA miR-504

Characterization of GDF11 signalling through type I and type II receptors. Characterization of GDF11 signalling through type I and type II receptors. Gene.

Expression of Hox genes, ALK5, GDF11 and ALK4 in Alk5−/− mutants.

Human embryonic kidney (HEK293T) cells expressing mutated glucose transporter 1-flag gene (MUT-GLUT1-flag) uptake less glucose than their WT-GLUT1-flag.

Loss of miRNA from Ago2 deactivates miRNPs in LPS‐treated mammalian macrophages. Loss of miRNA from Ago2 deactivates miRNPs in LPS‐treated mammalian macrophages.

NLK positively regulates YAP activity in physiological settings

Interaction between the G6PC2 rs G and rs G alleles on G6PC2 promoter activity. βTC-3 cells were transiently cotransfected, as described.

GA blocks HIF activity and reduces HIF target expression.

Presentation transcript:

NS3 inhibits endogenous miRNA action. NS3 inhibits endogenous miRNA action. HEK293T cells were co‐transfected with expression vectors encoding pCMV‐luc‐miR30‐P (left panel) or pCMV‐luc‐random (right panel), in combination with MBP, MBP‐NS3 or MBP‐NS3m (600 ng). Luciferase expression was measured 2 days post‐transfection and relative luciferase expression (firefly/Renilla) was determined. The luciferase level measured with NS3m was set at 1.0. HEK, human embryonic kidney; MBP, maltose‐binding protein; miRNA, microRNA; NS3, non‐structural protein 3; NS3m, mutant protein. Esther Schnettler et al. EMBO Rep. 2009;10:258-263 © as stated in the article, figure or figure legend