Lab Exercise 17: Biochemical Differentiation of some Medically Important Gram-negative Bacilli.

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Presentation transcript:

Lab Exercise 17: Biochemical Differentiation of some Medically Important Gram-negative Bacilli

MacConkey Agar Left: Shigella - result Right: Escherichia + result

Carbohydrate Fermentation Tests Glucose w/ Durham Fermentation Tube

Carbohydrate Fermentation Tests Sucrose Fermentation

Triple Sugar Iron -/+/+

IMViC Series Indole Test broth with tryptophan

IMViC Series Methyl Red Test

IMViC Series Voges-Proskauer Test  Leave uncapped for 15-20 minutes…….. MRVP broth

Citrate Utilization Test IMViC Series Citrate Utilization Test - +

Urease Test Slow positive Negative result (acidic) Uninoculated urea agar Positive result (alkaline)

Motility Test

A B 2. A 4. B A 3. B Answers: MacConkey agar 1. Which plate could have E. coli on it? 2. Which plate shows the presence of a lactose-fermenter? 3. Which plate could have Salmonella on it? 4. Which plate shows the presence of a lactose non-fermenter? A B 2. A 4. B A 3. B Answers:

What is the name of the lab procedure demonstrated here? Answer: Stabbing the butt!

C U I M V Answer: Shigella sonnei Use the chart on p. 181 and or the flow chart on p. 182 of your lab manual to figure out who I am! I M V C U Answer: Shigella sonnei

C I M V U Answer: Escherichia coli Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am! Answer: Escherichia coli

Answer: Serratia marcesens V C U Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am! Answer: Serratia marcesens

Answer: Klebsiella pneumoniae Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am! I M V C Answer: Klebsiella pneumoniae

Answer: Salmonella typhimurium Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am! I M V C U Answer: Salmonella typhimurium

Answer: Proteus vulgaris Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am! I M V C U Answer: Proteus vulgaris

Answer: Enterobacter aerogenes U Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am! I M V C Answer: Enterobacter aerogenes U