Glucose withdrawal activates a positive feedback loop resulting in supra‐physiological phospho‐tyrosine signaling and ROS‐mediated cell death. Glucose.

Slides:

Advertisements

Similar presentations

Oxidative Stress and Diabetes Jian Li Beijing Institute of Geriatrics Ministry of Health.

Advertisements

Statistical colorings of 1H NMR estimates of biochemical variables.

A stochastic feedback loop model predicts the kinetics of DDR and growth arrest at the single cell level. A stochastic feedback loop model predicts the.

PTP catalytic mechanism

Abundance of proteins matching selected subcellular locations and functions in CaCo‐2 cells. Abundance of proteins matching selected subcellular locations.

Schematic diagram of the three circuits analysed in this work.

Network topology reflects target gene expression profile and function.

A kinetic model reconciles observed ERK phosphorylation, localization, and activity responses A Schematic of a simple kinetic model including cytosolic.

Overview of the experimental setting.

Glucose withdrawal induces supra‐physiological levels of tyrosine phosphorylation in cells sensitive to glucose withdrawal. Glucose withdrawal induces.

Do reactive oxygen species play a role in myeloid leukemias?

(A) gToW growth phenotypes occur in the absence of leucine, and red squares indicate a significant growth‐rate defect as compared with the empty plasmid.

Illustration of the reactions and metabolites obtained from random sampling. Illustration of the reactions and metabolites obtained from random sampling.

Activity flow of the mTOR signaling network.

Figure 1 Immune cell metabolism during homeostasis

Oxidative stress in Alzheimer's disease

ROS Function in Redox Signaling and Oxidative Stress

Figure 4 Altered metabolism in chondrocytes in osteoarthritis

Confirmation of pool data with isogenic culture

Luminex phosphorylation measurements are reproducible and have broad dynamic range Individual difference in insulin‐induced phosphorylation of ERK measured.

Correlating protein to mRNA and proteins per mRNA ratios

Supplement Figure 3 SYF cells

Glutamine mediates oncogenic transformations in high‐invasive cells by regulating STAT3 activity Comparison of OVCAR3 invasive capacity in Gln depleted.

Martin D. Brand, Telma C. Esteves Cell Metabolism

Ashok Kumar Jayavelu, Jennifer N. Moloney, Frank-D. Böhmer, Thomas G

Networks of ERK substrates.

Physical EGFR interactome generation and core network proteins identification. Physical EGFR interactome generation and core network proteins identification.

Oxidative Modification of Leukocyte Adhesion

Chapter 14 Blood Pressure – Regulation and Pathology

The limiting, degrading entity hypothesis

Feature‐based COG enrichment analysis

Target identification of ampicillin and ciprofloxacin

(M9) WPDxGxP (M8) TxxD FWxMxW (M5) (M2) (M1) IAxQGP (M4) QTxx (M10)

Effect of the loss of Kar4 on the induction of various promoters

Schematic diagram of the proposed model depicting the functional relationship between EGFR and FASN in TKI resistance within EGFR mutant NSCLC cells Schematic.

Characterization of Factor 5 (oxidative stress response factor) in the CLL data Characterization of Factor 5 (oxidative stress response factor) in the.

Chemical inhibitors of microtubule function attenuate signal but do not affect pathway variability Chemical inhibitors of microtubule function attenuate.

Neurogenins induce a network of transcription factors that mediate iNGN neurogenesisA network of transcription factors involved in iNGN neurogenesis was.

Schematic summary of experimental findings.

Notch activation in Notch1 positive feedback knock‐out intestine organoids Notch activation in Notch1 positive feedback knock‐out intestine organoids Intestine.

p38δ and PKD1: Kinase Switches for Insulin Secretion

Allele‐specific expression at single‐cell resolution

Oxidative damage cell death pathway model.

Transcriptomic and epigenetic changes associated with Factor 1 in the scMT data Transcriptomic and epigenetic changes associated with Factor 1 in the scMT.

SRC and STAT Pathways Journal of Thoracic Oncology

Feedback signalling through TP53‐CDKN1A‐GADD45A‐MAPK14‐GRB2‐TGFBRII‐TGFβ induces ROS production and maintains DDR. (A–C) MRC5 cells were transfected with.

Volume 83, Issue 4, Pages (April 2013)

Direct role of HIS-24 and HPL in stress response.

Live‐cell imaging uncovers a slowly cycling drug‐resistant state involved in adaptation to RAF inhibition Live‐cell imaging uncovers a slowly cycling drug‐resistant.

Glutamine has pleiotropic role of positively regulating respiration and maintaining redox balance selectively in high‐invasive ovarian cancer (OVCA) cells.

Simulation showing that the cell length variability of the entire population can mask abnormal cell length variability at a specific cell cycle period.

Kinome‐wide activity regulation derived from known substrates and 41 quantitative phosphoproteomic studies Kinome‐wide activity regulation derived from.

An integrated NHR network.

Feedback loop signalling is necessary and sufficient to maintain proliferation arrest during establishment of irreversible cell senescence. Feedback loop.

When grown with biofuel, strains with beneficial pumps dominate the culture. When grown with biofuel, strains with beneficial pumps dominate the culture.

Maintenance metabolism alone cannot explain non‐division

Melting behavior of protein complexes

Evolution of Mitochondria as Signaling Organelles

Experimental validation of predicted endocytosis functions in human.

Hydrogen Peroxide Sensing and Signaling

Interaction of Gpr1/Gpa2 and Sch9.

Glucose pulses incorporate directly into the de novo biomass in non‐dividing cells Glucose pulses incorporate directly into the de novo biomass in non‐dividing.

MAPK14 regulates ROS generation in GS cells.

DuMPLING oligonucleotide plasmid library design and production

Oscillations in isotopic labeling of TCA cycle metabolites throughout the cell cycle from [U‐13C]‐glucose show induced glycolytic flux into TCA cycle in.

Constantijn Franssen et al. JCHF 2016;4:

O2 is transported from atmospheric air to the mitochondria of tissue cells along a pathway with several diffusive and convective steps. O2 is transported.

Summary of the new mechanistic findings of the present study.

The flux of glucose from the blood to the membrane to muscle.

Effect of IS on proliferation, senescence, and the production of NO and ROS from endothelial cells. Effect of IS on proliferation, senescence, and the.

Presentation transcript:

Glucose withdrawal activates a positive feedback loop resulting in supra‐physiological phospho‐tyrosine signaling and ROS‐mediated cell death. Glucose withdrawal activates a positive feedback loop resulting in supra‐physiological phospho‐tyrosine signaling and ROS‐mediated cell death. In cells dependent on glucose for survival, glucose and pyruvate deprivation induces oxidative stress driven by NOX and mitochondria. This oxidative stress provokes a positive feedback loop in which NOX and mitochondria generate superoxide anion (O2−), which dismutes to hydrogen peroxide (H2O2) and inhibits PTPs by oxidation (e.g., PTP‐1B and PTEN). Without the negative regulation of PTPs, TKs including EGFR and Src activate NOX at focal adhesions, further amplifying ROS generation. This glucose withdrawal‐induced positive feedback loop results in supra‐physiological levels of tyrosine phosphorylation and ROS‐mediated cell death. Nicholas A Graham et al. Mol Syst Biol 2012;8:589 © as stated in the article, figure or figure legend