Glutamine Suppresses DNFB-Induced Contact Dermatitis by Deactivating p38 Mitogen– Activated Protein Kinase via Induction of MAPK Phosphatase-1 Otgonzaya.

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Presentation transcript:

Glutamine Suppresses DNFB-Induced Contact Dermatitis by Deactivating p38 Mitogen– Activated Protein Kinase via Induction of MAPK Phosphatase-1 Otgonzaya Ayush, Chang-Hoon Lee, Hae-Kyoung Kim, Suhn-Young Im, Baik-Hwan Cho, Hern-Ku Lee Journal of Investigative Dermatology Volume 133, Issue 3, Pages 723-731 (March 2013) DOI: 10.1038/jid.2012.373 Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 L-glutamine (Gln) suppresses DNFB-induced contact dermatitis. (a) Ear swelling responses (ESRs) were measured. (b) After measurement of ESR at 24hours, ear sections from the (A) vehicle, (B) DNFB, (C) DNFB+Ala (× 5), (D) DNFB+Gln (× 1), (E) DNFB+Gln (× 3), or (F) DNFB+Gln (× 5)-treated group were stained with hematoxylin and eosin (H&E). (c) Cytokine levels were measured and calculated as picograms of protein per milligram of homogenized tissue. (d) ESR at 24hours after challenge with oxazolone (Ox) or picryl chloride (PCl). (e) After measurement of ESR, ear sections from the (A) Ox vehicle, (B) Ox, (C) Ox+Gln (× 5), (D) PCl vehicle, (E) PCl, or (F) PCl+Gln (× 5)-treated group were stained with H&E. Data in a, c, and d represent the mean±SD of three independent experiments with five mice per group. *P<0.05; **P<0.01. TNF, tumor necrosis factor. Bar=100μm. Journal of Investigative Dermatology 2013 133, 723-731DOI: (10.1038/jid.2012.373) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Gln inhibits p38 and c-Jun N-terminal kinase (JNK) phosphorylation. The ears were removed at the indicated time points after DNFB challenge. Protein levels and phosphorylation of mitogen-activated protein kinases were determined by western blotting and quantified by densitometric analysis. A representative of three to five independent experiments with 3–5 mice per time point per experiment is shown. ERK, extracellular signal–regulated kinase; p-ERK, phosporylated ERK; p-JNK, phosporylated JNK. Journal of Investigative Dermatology 2013 133, 723-731DOI: (10.1038/jid.2012.373) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 p38 inhibitor attenuates DNFB-induced contact dermatitis. (a) Mitogen-activated protein kinase inhibitors were injected intraperitoneally (i.p.) 24hours before the challenge of DNFB. Ear swelling responses (ESRs) were measured at the indicated time points. (b) After measurement of ESR at 24hours, the mice were killed, and ear sections from the (A) vehicle (DMSO), (B) DNFB, (C) DNFB+U0126, (D) DNFB+SP600125, or (E) DNFB+SB202190-treated group were stained with hematoxylin and eosin. (c) Ear biopsy homogenates were taken 24hours after the challenge and cytokine levels were measured using ELISA and calculated as picograms of protein per milligram of homogenized tissue solution. Data in a and c represent the mean±SD of three independent experiments with five mice per group. *P<0.05; **P<0.01. TNF, tumor necrosis factor. Bar=100μm. Journal of Investigative Dermatology 2013 133, 723-731DOI: (10.1038/jid.2012.373) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 L-glutamine (Gln) deactivates p38 via mitogen-activated protein kinase phosphatase (MKP)-1. (a) Gln or Ala was applied onto the ears. (b) Mice received small interfering RNA (siRNA) 24hours before challenge of DNFB. The ears were removed 90minutes after challenge. (c) Mice were pretreated with siRNA, and Gln was applied three times (× 3) after DNFB challenge. Mice were killed at 50minutes. (d) HaCaT cells were stimulated with DNFB (5μgml−1). Gln (20, 40, or 80mM) was added to the HaCaT cells (2 × 106) 10 min after DNFB stimulation. (e) Gln (80mM) was added at 10minutes and the cells were harvested at the times indicated. A representative of three to five independent experiments with 3–5 mice per time point per experiment is shown. Journal of Investigative Dermatology 2013 133, 723-731DOI: (10.1038/jid.2012.373) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Mitogen-activated protein kinase phosphatase (MKP)-1 small interfering RNA (siRNA) abrogates the suppressive activity of L-glutamine (Gln) against DNFB-induced contact dermatitis. Mice were intravenously injected with 200μl of the polyethyleneimine mixture containing 0.4nmol of siRNA 24hours before challenge of DNFB. Gln was applied three times (× 3) in the ear skin after DNFB challenge. (a) Measurement of ear swelling response (ESR), (b) hematoxylin and eosin (H&E) staining from mice that received (A) vehicle, (B) DNFB, (C) DNFB+Gln (× 3), (D) DNFB+Gln (× 3)+MKP-1 siRNA, or (E) DNFB+Gln (× 3)+control siRNA, and (c) measurement of cytokine levels in the ear tissues were performed. Data in a and c represent the mean±SD of three independent experiments with five mice per group. *P<0.05; **P<0.01. TNF, tumor necrosis factor. Bar=100μm. Journal of Investigative Dermatology 2013 133, 723-731DOI: (10.1038/jid.2012.373) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 6 Extracellular signal–regulated kinase (ERK) inhibitor reverses the effects of Gln on mitogen-activated protein kinase phosphatase (MKP)-1 induction, p38 phosphorylation, and ear inflammation. ERK inhibitor U0126 was administered intraperitoneally 24hours before DNFB challenge. Gln was applied three times (× 3) in the ear skin after DNFB challenge. (a) ERK phosphorylation, (b) MKP-1 phosphorylation, (c) MKP-1 protein induction and p38 phosphorylation, and (d) measurement of ear swelling response were performed, as well as (e) histological examination of groups that received (A) vehicle, (B) DNFB, (C) DNFB+Gln (× 3), or (D) DNFB+Gln (× 3)+U0126. Data in a, b, and c are representative of three to five independent experiments with 3–5 mice per time point per experiment. Data in d represent the mean±SD of three independent experiments with five mice per group. **P<0.01. Bar=100μm. Journal of Investigative Dermatology 2013 133, 723-731DOI: (10.1038/jid.2012.373) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions