Surveillance of Foodborne Enteropathogen in Brazil

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Presentation transcript:

Surveillance of Foodborne Enteropathogen in Brazil National Reference Laboratory Laboratory of Enterobacteria Oswaldo Cruz Institut-FIOCRUZ Dalia dos Prazeres Rodrigues, Ph.D

Ministry of Health Secretary of Health Surveillance National Reference Laboratory Regional Reference Laboratories State Reference Laboratories (PHL) Municipal Labs Border Labs Local Labs National Epidemiological Surveillance Network National Environmental Surveillance Network National Sanitary Surveillance Network National Health Assistance Network Collaborating Centre National Division

Regional laboratory

Public Health Laboratories Ministry of Health- Laboratories Coordination National Reference Laboratory IOC / FIOCRUZ Exotic serovars Multidrug resistant strains Epidemic strains Surveillance of emergent species/serovars Public Health Laboratories

National Reference Laboratory-activities Sampling: Human source and foodborne outbreaks by PHL; S.Typhi, V.cholerae O1 and non O1, Aeromonas sp. and, V.parahaemolyticus strains; All Salmonella spp. isolates from animal, feed and foodstuff products by Ministry of Agriculture Laboratories; Serotyping activities-Salmonella spp: Isolates from Public and Private Universities; Isolates from private Institutions from Food, Animal, Feed, Environment, and Foodstuff ; Activities carried out by NRL Serotyping; Salmonella spp., Shigella sp. , E.coli, V.parahamolyticus, Listeria monocytogenes, Surveillance of antimicrobial susceptibility and molecular characterization; Phage typing: S.Typhi, S.Typhimurium, S.Enteritidis, S.Hadar; PCR- virulence genes Sub typing - PFGE

Total strains isolated from human source received from PHL 2001 2002 2003 2004 2005 2006 8,161 9,826 10,679 13,770 17,495 17,020

Activities carried out by NRL-2006-2007 PFGE Shigella flexneri: Total analyzed strains: 50

Pulsenet activities Salmonella Enteritidis Salmonella Typhimurium Salmonella 1,4,12:i:-

V.parahaemolyticus Enzyme: Not I Total analyzed strains: 35 PE 2001 12 State Ano Strains No. Serovars PCR Kanagawa Tdh PE 2001 12 O3:KUN + AL 2002 8 O3:K6 CE 18 O3 K6

V.parahaemolyticus

Activities carried out by NRL- PFGE: 2006-2007 Outbreak Surveillance S.Panama (>185 human cases) State: Pernambuco (total: 8 strains) S.Enteritidis (32 human cases) State : Bahia (total: 14 strains) S. Enteritidis (? Human cases) State: Roraima (total: 13 strains) S.Typhimurium DT120 (> 50 human cases) State: Rio de Janeiro (total: 5 strains) S.Enteritidis (human, food, animal) Total analyzed strains: 35 S.Heidelberg (food) Total analyzed strains: 15 S.Typhimurium DT 104, DT120, DT193 (human, food, animal, feed) Total analyzed strains: 40 S. Agona (human, food, feed, animal) Total analyzed strains: 60 Salmonella spp. strains isolated from sewage waters in Pan American Ville

S.Panama S.Agona

Estimated activities: 2007-2008 GSS-Level II course- strengthen the PHL into classical and PCR diagnostic of Campylobacter sp. and antigenic diagnostic of Salmonella spp. and other enteropathogens PFGE course to Regional Laboratories; Implemented activities between National Reference Laboratory/IOC and Biomanguinhos/FIOCRUZ providing polyvalent and monovalent antisera; Standardize PFGE to Aeromonas hydrophila; Standardize PFGE to Listeria monocytogenes Attend the collaborative Pulsenet activities;