Cartilage Repair in the Knee Using Umbilical Cord Wharton's Jelly–Derived Mesenchymal Stem Cells Embedded Onto Collagen Scaffolding and Implanted Under.

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Cartilage Repair in the Knee Using Umbilical Cord Wharton's Jelly–Derived Mesenchymal Stem Cells Embedded Onto Collagen Scaffolding and Implanted Under Dry Arthroscopy Boguslaw Sadlik, M.D., Ph.D., Grzegorz Jaroslawski, M.D., Mariusz Puszkarz, M.D., Adrian Blasiak, M.D., Ph.D., Tomasz Oldak, Ph.D., Dominika Gladysz, M.D., Graeme P. Whyte, M.D., M.Sc. Arthroscopy Techniques Volume 7, Issue 1, Pages e57-e63 (January 2018) DOI: 10.1016/j.eats.2017.08.055 Copyright © 2017 Arthroscopy Association of North America Terms and Conditions

Fig 1 Wharton's jelly–derived mesenchymal stem cell preparation. (A) Human umbilical cord measuring 10 cm in length (arrow). (B) Histologic slide of sectioned umbilical cord with hematoxylin and eosin staining (magnification ×20). (C) Contrast image of Wharton's jelly–derived mesenchymal stem cells after expansion in xeno-free medium. Arthroscopy Techniques 2018 7, e57-e63DOI: (10.1016/j.eats.2017.08.055) Copyright © 2017 Arthroscopy Association of North America Terms and Conditions

Fig 2 (A) Preparation of cartilage lesion located at medial trochlea and lateral aspect of medial femoral condyle, with patient supine and arthroscope positioned in anterolateral portal. (B) Sterile dental dam used for templating of lesion. (C) Size matching of type I/III collagen scaffold (arrow) to prepared defect. (D) Type I/III collagen scaffold (arrow) saturated with Wharton's jelly–derived mesenchymal stem cell suspension. (E) Specialized graft-inserting instrument used to simplify arthroscopic implant delivery through anteromedial portal. (F) Dry arthroscopic application of type I/III collagen scaffold embedded with Wharton's jelly–derived mesenchymal stem cells (WJ-MSCs) onto chondral defect of medial trochlea and lateral aspect of medial femoral condyle, with visualization through anterolateral portal. Arthroscopy Techniques 2018 7, e57-e63DOI: (10.1016/j.eats.2017.08.055) Copyright © 2017 Arthroscopy Association of North America Terms and Conditions

Fig 3 Proton density–weighted magnetic resonance images (coronal and sagittal views) showing a grade III to IV cartilage lesion of the medial femoral condyle (arrows) preoperatively (A, B); Wharton's jelly–derived mesenchymal stem cell–embedded scaffold (arrows) 6 months after dry arthroscopic implantation (C, D); and Wharton's jelly–derived mesenchymal stem cell–embedded scaffold (arrows) 9 months after implantation, depicting integration of regenerative tissue with surrounding cartilage and subchondral lamina (E, F). Arthroscopy Techniques 2018 7, e57-e63DOI: (10.1016/j.eats.2017.08.055) Copyright © 2017 Arthroscopy Association of North America Terms and Conditions