Polyethylene wear particles play a role in development of osteoarthritis via detrimental effects on cartilage, meniscus, and synovium D.Y. Park, B.-H.

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Presentation transcript:

Polyethylene wear particles play a role in development of osteoarthritis via detrimental effects on cartilage, meniscus, and synovium D.Y. Park, B.-H. Min, D.-W. Kim, B.R. Song, M. Kim, Y.J. Kim Osteoarthritis and Cartilage Volume 21, Issue 12, Pages 2021-2029 (December 2013) DOI: 10.1016/j.joca.2013.09.013 Copyright © 2013 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 (A) Microscopic view of the silicon wafer surface. Each micro-cutting edge was 5 μm apart, with 1.3 μm in height. (B) SEM of UHMWPE wear particles generated by micro-cutting process. Osteoarthritis and Cartilage 2013 21, 2021-2029DOI: (10.1016/j.joca.2013.09.013) Copyright © 2013 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Dot plot representing concentration of inflammatory cytokines IL-1β, IL-6, TNF-α, and inflammatory mediators PGE2 and NO after co-culture of chondrocytes, meniscal fibrochondrocytes, and synoviocytes with UHMWPE. Differences among groups with P ≤ 0.05 are enumerated. Osteoarthritis and Cartilage 2013 21, 2021-2029DOI: (10.1016/j.joca.2013.09.013) Copyright © 2013 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Phagocytosis index analysis by flow cytometry. Dot plot represents the percentage of cells showing phagocytic activity, determined by increase of side scatter by FACS analysis. Differences among groups with P ≤ 0.05 are enumerated. Osteoarthritis and Cartilage 2013 21, 2021-2029DOI: (10.1016/j.joca.2013.09.013) Copyright © 2013 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Confocal microscopy of chondrocyte, meniscal fibrochondrocyte, and synoviocyte co-cultured with UHMWPE. Images of PI stained cells were merged with FITC fluorescence images. UHMWPE particles, which show FITC fluorescence (white arrows), are seen inside each cell type, suggestive of phagocytosis. Chromatin condensation, are noted in all cell types, suggestive of apoptotic activity (A) Chondrocytes showing engulfment of UHMWPE. (B) Meniscal fibrochondrocytes showing engulfment of UHMWPE. (C) Synoviocytes showing engulfment of UHMWPE. Note the membrane blebbing, suggestive of apoptotic activity. x600. Osteoarthritis and Cartilage 2013 21, 2021-2029DOI: (10.1016/j.joca.2013.09.013) Copyright © 2013 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 Apoptosis after co-culture of intra-articular cells and UHMWPE by TUNEL assay. The percentage of TUNEL positive cells are shown in the dot plot. Differences among groups with P ≤ 0.05 are enumerated. Osteoarthritis and Cartilage 2013 21, 2021-2029DOI: (10.1016/j.joca.2013.09.013) Copyright © 2013 Osteoarthritis Research Society International Terms and Conditions

Fig. 6 Representative histopathological results of all groups. Upper row shows cartilage slides, stained in SAFO. Meniscus slides, also stained in SAFO are shown in the middle row. Synovium slides, stained in HE, are shown in the lower row. X200. Osteoarthritis and Cartilage 2013 21, 2021-2029DOI: (10.1016/j.joca.2013.09.013) Copyright © 2013 Osteoarthritis Research Society International Terms and Conditions

Fig. 7 (A) Quantitative representation of cartilage status by OARSI scoring. (B) Quantitative representation of degenerative changes of menisci. (C) Quantitative representation of synovitis. Error bars represent 95% confidence intervals. Differences among groups with P ≤ 0.05 are enumerated. Osteoarthritis and Cartilage 2013 21, 2021-2029DOI: (10.1016/j.joca.2013.09.013) Copyright © 2013 Osteoarthritis Research Society International Terms and Conditions

Osteoarthritis and Cartilage 2013 21, 2021-2029DOI: (10. 1016/j. joca Copyright © 2013 Osteoarthritis Research Society International Terms and Conditions