Inhibition of micro-ribonucleic acid-320 attenuates neurologic injuries after spinal cord ischemia Fang He, MD, Enyi Shi, MD, PhD, Lihui Yan, MD, Juchen.

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Inhibition of micro-ribonucleic acid-320 attenuates neurologic injuries after spinal cord ischemia Fang He, MD, Enyi Shi, MD, PhD, Lihui Yan, MD, Juchen Li, MD, Xiaojing Jiang, MD, PhD The Journal of Thoracic and Cardiovascular Surgery Volume 150, Issue 2, Pages 398-406 (August 2015) DOI: 10.1016/j.jtcvs.2015.03.066 Copyright © 2015 The American Association for Thoracic Surgery Terms and Conditions

Figure 1 Experimental groups and protocol. miR-320, Micro-ribonucleic acid-320; phospho-Hsp20, phosphorylation state of heat-shock protein 20; TU, transfection units; antagomiR-320, antisense oligonucleotides of rat miR-320. The Journal of Thoracic and Cardiovascular Surgery 2015 150, 398-406DOI: (10.1016/j.jtcvs.2015.03.066) Copyright © 2015 The American Association for Thoracic Surgery Terms and Conditions

Figure 2 Time course of expressions of miR-320 and phospho-Hsp20 in the spinal cord after ischemia. A, Representative western blot picture showing phospho-Hsp20 expression. B, Densitometric quantification of phospho-Hsp20 expression. C, Densitometric quantification of miR-320 expression; n = 4 in each group. phospho-Hsp20, Phosphorylation state of heat-shock protein 20; miR-320, micro-ribonucleic acid-320. *P < .01, compared with the sham group. The Journal of Thoracic and Cardiovascular Surgery 2015 150, 398-406DOI: (10.1016/j.jtcvs.2015.03.066) Copyright © 2015 The American Association for Thoracic Surgery Terms and Conditions

Figure 3 Effects of antagomiR-320 administration on expression of miR-320 and phospho-Hsp20 in the spinal cord, 2 hours after reperfusion. A, Representative western blot picture showing phospho-Hsp20 expression. B, Densitometric quantification of phospho-Hsp20 expression. C, Densitometric quantification of miR-320 expression; n = 4 in each group. phospho-Hsp20, Phosphorylation state of heat shock protein 20; AntagomiR-320, antisense oligonucleotides of rat miR-320; miR-320, micro-ribonucleic acid-320. *P < .01, compared with the control group. The Journal of Thoracic and Cardiovascular Surgery 2015 150, 398-406DOI: (10.1016/j.jtcvs.2015.03.066) Copyright © 2015 The American Association for Thoracic Surgery Terms and Conditions

Figure 4 Effects of antagomiR-320 administration on hind-limb motor function, assessed using the Motor Deficit Index, before spinal cord ischemia, and 6, 12, 24, and 48 hours after reperfusion. Solid triangles indicate the group of antagomiR-320. *P < .01, compared with the control group. AntagomiR-320, Antisense oligonucleotides of rat miR-320. The Journal of Thoracic and Cardiovascular Surgery 2015 150, 398-406DOI: (10.1016/j.jtcvs.2015.03.066) Copyright © 2015 The American Association for Thoracic Surgery Terms and Conditions

Figure 5 Effects of antagomiR-320 administration on neuron survival after spinal cord ischemia. A, Representative sections of lumbar spinal cord, stained with hematoxylin-eosin (a-d) and Nissl dye (e-h): sham group (a, e); control group (b, f); control vector group (c, g); antagomiR-320 group (d, h). B, Number of large motor neurons in the ventral gray matter of lumber spinal cord. AntagomiR-320, Antisense oligonucleotides of rat miR-320. *P < .01, compared with the control group. The Journal of Thoracic and Cardiovascular Surgery 2015 150, 398-406DOI: (10.1016/j.jtcvs.2015.03.066) Copyright © 2015 The American Association for Thoracic Surgery Terms and Conditions

Inhibition of miR-320 protects motor function after spinal cord ischemia. AntagomiR-320, Antisense oligonucleotides of rat miR-320. *P < .01, compared with the control group. The Journal of Thoracic and Cardiovascular Surgery 2015 150, 398-406DOI: (10.1016/j.jtcvs.2015.03.066) Copyright © 2015 The American Association for Thoracic Surgery Terms and Conditions