PtdIns5P promotes cell migration.

Slides:

Advertisements

Similar presentations

Effects of inhibiting inflammatory cell recruitment on fracture healing Treatment with CCR2 antagonist, INCB3344, led to impaired fracture healing compared.

Advertisements

ABAE positive cells 6h after treatment

Validating Cdk‐dependent chromatin association changes of selected outliers A–EValidation of changes in chromatin affinity following Cdk inhibition of.

Cycloheximide inhibits arsenite‐induced stress granule formation and prevents the decreased number of senescent cells mediated by repeated arsenite treatment.

Induction of mPer1 mRNA expression by prostaglandin E2 (PGE2) in NIH3T3 cells. Induction of mPer1 mRNA expression by prostaglandin E2 (PGE2) in NIH3T3.

NS3 has no interferon and PKR antagonistic properties.

Cdc42.GTP directly activates PAK4.

Nur77 interacts with COUP‐TF in yeast.

An siRNA screen identifies LIMT as an ERK‐dependent negative regulator of mammary cell migration An siRNA screen identifies LIMT as an ERK‐dependent negative.

The cyclin B1 promoter is accessible to restriction endonucleases in vivo. The cyclin B1 promoter is accessible to restriction endonucleases in vivo. Nuclei.

JNK is activated by Wnt5a.

H1‐NPCs differentiate into TH+ midbrain DA neurons with high efficiency. H1‐NPCs differentiate into TH+ midbrain DA neurons with high efficiency. (A) MAP2‐expressing.

HIF1 binds and stimulates the VEGFA promoter in normoxia.

Downregulation of ΔNp73 leads to re‐activation of p53‐regulated genes and apoptosis. Downregulation of ΔNp73 leads to re‐activation of p53‐regulated genes.

Effects of iron chelation on mitochondrial function.

Loss of USP8 or HIF1α impairs endocytic recycling required for ciliogenesis Kinetic analysis of transferrin recycling measured by flow cytometry. Loss.

Induction of apoptosis by CDIP expression.

Tie1 silencing‐induced endothelial–mesenchymal transition is Slug dependent. Tie1 silencing‐induced endothelial–mesenchymal transition is Slug dependent.

Set9 inhibits oxidative stress‐induced neuronal cell death via methylation of FOXO3. Set9 inhibits oxidative stress‐induced neuronal cell death via methylation.

Trx80 is generated by α‐secretases. A

Mitophagy is independent of PINK1 and Parkin.

TRF2ΔBΔM induces apoptosis and senescence in the mouse liver.

Transcriptional activity of the exogenous cyclin B1 promoter is high in the G2/M phases of the cell cycle. Transcriptional activity of the exogenous cyclin.

Subcellular localization of full‐length and cleaved PINK1 PINK1 immunoblot of cytoplasmic and mitochondrial fractions from HeLa cells transfected with.

Overexpression of RNase H1 decreases the induction of phosphorylated H2AX in post‐mitotic cells in response to topoisomerase 1 cleavage complexes. Overexpression.

c‐Abl acetylation on Lys 730 promotes myogenic differentiation.

Non‐specific activation of gene transcription by exogenous Fe65 in luciferase‐based reporter assays. Non‐specific activation of gene transcription by exogenous.

Interaction with Snail1 is required for LSD1 recruitment to the E‐cadherin promoter in vivo. Interaction with Snail1 is required for LSD1 recruitment to.

NoRC regulates telomeric heterochromatin.

Direct transcriptional regulation oflncRNAs by HIF AHeat map showing fold regulation by hypoxia and by the indicated HIF siRNA for lncRNAs adjacent to.

Both NM1 and the WSTF complex stimulate Pol I transcription.

Mutations affecting HOIP–UBA and HOIL‐1L–UBL interaction that compromise NF‐κB activation. Mutations affecting HOIP–UBA and HOIL‐1L–UBL interaction that.

Roles of systemic versus tumour SK1 in the regulation of local tumour growth and lung colonization/metastasis.A.Effects of siRNA‐mediated SK1 knockdown.

Fstl1 promotes cardiac fibroblasts proliferation via the ERK1/2‐dependent pathway Fstl1 promotes cardiac fibroblasts proliferation via the ERK1/2‐dependent.

Quantitative mass spectrometry of proteasome pulldowns

NoRC affects centromere function.

ADAM8 maintains the aggressive phenotype of TNBC cells in 3D‐culture A–DCells were transfected with siRNAs as in Fig 2D and E for 24 h, and tested for.

HS induces sustained H3K4me3 and H3K4me2 methylation at HSP22

Triple‐negative breast cancer (TNBC) cells in human express a specific gene signature and their self‐renewal depends on canonical Wnt/β‐catenin signalling.A.Human.

EphrinB2/EphB4 signaling regulates the degree of vascular enlargement by VEGF dose EphrinB2/EphB4 signaling regulates the degree of vascular enlargement.

ECM 29 and Kaplan–Meier survival curves for additional datasets

Palbociclib activates the proteasome indirectly and reduces the association of ECM29 with the proteasome Palbociclib activates the proteasome indirectly.

RASGRP1 is required for T‐cell expansion in response to CD3‐TCR activation RASGRP1 is required for T‐cell expansion in response to CD3‐TCR activation Rescue.

USP30 restricts basal pexophagy

PEX13 is required for Sindbis virophagy but not general autophagy

Characterization of oligo#5 and its derivatives

Beclin‐1 depletion reduces targeting of several outer kinetochore proteins. Beclin‐1 depletion reduces targeting of several outer kinetochore proteins.

NRP2 induces expression of GLI1. A,B

SEMA3C silencing inhibits CRPC cell growth and induces apoptosis

WDR11 cooperates with Hh pathway to regulate gene expression

Mitochondrial ceramide is involved in HPV‐E7‐mediated lethal mitophagy

Endogenous MOSPD2 is recruited to interorganelle contact sites by FFAT‐containing proteins Endogenous MOSPD2 is recruited to interorganelle contact sites.

Myc increases the instability of lac operator repeats.

Deficient segregation of the lac operator insertion.

Activated Gα13 inhibits AhR‐mediated transcriptional activity.

Histone deacetylase 1 protein depletion affects general histone acetylation and specific gene expression. Histone deacetylase 1 protein depletion affects.

MOSPD2 silencing affects interorganelle contact sites organization

The ERK pathway mediates the effect of EGF on LIMT, which normally inhibits mammary cell migration and invasion The ERK pathway mediates the effect of.

IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice ARepresentative.

HDAC1 positively regulates the abundance of miRNAs.

MET inhibition promotes p21 nuclear translocation

A kinase‐dead mutant and an ECO‐associated mutant of ICK show a decreased ability to rescue shortened cilia phenotype in ICK−/− MEFs A–FKinase activity.

R1441G‐LRRK2 co‐localizes with Rab10 in the periphery and Rab8 near the nucleus R1441G‐LRRK2 co‐localizes with Rab10 in the periphery and Rab8 near the.

ZBTB48 is required for MTFP1 expression

Growth of TKI‐resistant EGFR delE746‐A750 PC‐9 NSCLC cells is associated with elevated cellular free fatty acid and expression of FASN, and is suppressed.

P2Y1 receptor mediated the directional migration of SVZ neuroblasts in an EF. (A) Suramin (general purinergic receptor inhibitor, 5 μM) did not affect.

NLK positively regulates YAP activity in physiological settings

Rab27a promotes migration of neutrophils in vivo and in vitro.

TSC1 knockdown enhances hypoxia-mediated lymphocyte death via mTORC1 activation TSC1 knockdown enhances hypoxia-mediated lymphocyte death via mTORC1 activation.

FBXL19-AS1 knockdown inhibits proliferation, migration and invasion, and reduces the expression levels of angiogenesis related proteins in lung cancer.

Presentation transcript:

PtdIns5P promotes cell migration. PtdIns5P promotes cell migration. (A) Stimulation of cell migration by FGF1. Quantification of BJ cell velocity in a wound healing assay without or after stimulation with FGF1 (200 ng ml−1). Cells analysed in total: 90 (without FGF1); 90 (with FGF1). (B) PtdIns5P levels (percentage of total phosphoinositides) in BJ cells stimulated with FGF1 and different siRNA treatments as indicated. Values are means±s.e.m. of five independent experiments. (C) Quantification of cell velocity on MTMR3 knockdown after treatment with exogenous phosphoinositides as indicated. Cells analysed in total: 225 (control siRNA); 225 (MTMR3 siRNA); 230 (rescue PtdIns5P); 150 (rescue PtdIns3P); 200 (rescue PtdIns(3,5)P2); 165 (rescue PtdIns4P); 145 (rescue PtdIns5P on control siRNA). (D) Quantification of cell velocity on PIKfyve knockdown after treatment with exogenous phosphoinositides as indicated. Cells analysed in total: 150 (control siRNA); 200 (PIKfyve siRNA); 170 (rescue PtdIns5P); 175 (rescue PtdIns3P); 205 (rescue PtdIns(3,5)P2). (E) Quantification of cell velocity after rescue with cDNA of the bacterial enzyme IpgD. Cells analysed in total: 65 (control siRNA); 40 (MTMR3 siRNA); 10 (rescue IpgD); 16 (rescue IpgD C438S); 23 (rescue IpgD on control siRNA). (F) Schematic representation of pathways for PtdIns5P synthesis. The production of PtdIns5P by the bacterial enzyme IpgD is also indicated. For all panels, error bars represent the mean±s.e.m. of three independent experiments. *P<0.05; **P<0.01; ***P<0.001; Student's t‐test. Angela Oppelt et al. EMBO Rep. 2013;14:57-64 © as stated in the article, figure or figure legend