Suppression of ENTPD5 in human NSCLC cells results in decreased AKT activity and decreased proliferation under low-serum conditions. Suppression of ENTPD5.

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Suppression of ENTPD5 in human NSCLC cells results in decreased AKT activity and decreased proliferation under low-serum conditions. Suppression of ENTPD5 in human NSCLC cells results in decreased AKT activity and decreased proliferation under low-serum conditions. A and D, levels of ENTPD5, IGF-IRβ, phospho-S473 AKT (p-AKT; high and low exposures), total AKT, PTEN, and GAPDH in A549 and NCI-H23 human NSCLC cell lines (A), or in NCI-H23 cells with stable knockdown of GFP or ENTPD5 (shE5-a; D), grown in media supplemented with 10% serum or 0% serum for 1 or 24 hours. B and E, graphs representing quantified changes (obtained by ImageJ) in ENTPD5, IGF-IRβ or PTEN, over GAPDH protein levels or p-AKT/AKT, assayed for in A or D, respectively. C and F, proliferation curves of A549 and NCI-H23 (C), described in A or NCI-H23-shGFP, NCI-H23-shE5-a cell lines (F), described in D, grown in the presence of 0.1% serum supplemented with increasing concentrations of insulin or IGF-I for 7 days (n = 6). **, P < 0.01; ****, P < 0.0001 (ANOVA/Bonferroni tests for differences between 1 ng/mL insulin or 0.5 ng/mL IGF-I and all other conditions at day 7 in C and F). In A, separated lanes of the ENTPD5 blot were acquired from a single electrophoresis gel. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. Natasha L. Curry et al. Cancer Discovery 2013;3:908-921 ©2013 by American Association for Cancer Research