Cocci. Cocci Bacilli Spirochetes Staphylococcus Arrangement.

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Presentation transcript:

Cocci

Bacilli

Spirochetes

Staphylococcus Arrangement

Diplococcus

Streptococcus

Streptobacillus Arrangement

raised, Whole Colony MARGIN ELEVATION round irregular filamentous rhizoid MARGIN Smooth, entire rhizoid Irregular (erose) lobate filamentous ELEVATION convex umbonate plateau flat raised, spreading edge flat raised margin growth into medium raised

Opaque Center and Spreading Edge

Shiny and Umbonate

Irregular and Rhizoid

Punctiform Colonies

Stains

Wet Mount (Hanging Drop)

Cheek Cells Without Stain

Cheek Cells with Simple Stain (Methylene Blue)

Simple Stain: Negative Stain

Negative Stain

Differential Stain: Gram Stain Color of Gram + Gram – Primary stain: Crystal violet Purple Mordant: Iodine Decolorizing agent: Alcohol-acetone Colorless Counterstain: Safranin Pink

Outer membrane Peptidoglycan GRAM NEGATIVE GRAM POSITIVE

Gram Positive and Gram Negative Bacteria

Gram Stain of Staphylococcus epidermidis (+) and Citrobacter diversus (-).

Acid-Fast Stain ZN Method

Acid-Fast and Non Acid-Fast

Endospore Stains

Endospores

Endospore Stain: Ziehl-Neelsen

Figure 3.12a-c

Endospore Stain

Stain Comparisons G+ (PURPLE) G – (PINK) ACID FAST PRIMARY STAIN ENDOSPORE STAIN PRIMARY STAIN Crystal violet Carbol Fuschia Malachite green MORDANT Iodine Heat DECOLORIZER ETOH or acetone: Cell is purple Cell is clear Acid alcohol Water COUNTER-STAIN Safranin: Cell is pink Methylene Blue Spores are

Capsule Stain

Capsule Stain

Figure 3.12a-c

Flagella Stain

Flagella Stain

Peritrichous Flagella

Figure 3.12a-c

Names for flagella arrangement Monotrichous Lophotrichous Amphitrichous Peritrichous

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