Practical 2 Gram staining.

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Presentation transcript:

Practical 2 Gram staining

Differential staining Acceptance of stains is an important property of bacteria and is the base divission of bacteria to 2 principal groups Gram positive and Gram negative in taxonomy. Gram staining

GRAM STAINING PROCEDURE Prepare a heat fixed smear of the culture you wish to examine Flood the smear with crystal violet (30 sec. to 2 min) Quickly and gently wash off excess stain (2 seconds) Flood the smear with Grams iodine (1 minute) Decolorize with alcohol (10-20 seconds or until the excess alcohol which flow off the slide is colorless) Flood the smear with safranin (carbolfuchsin) (30 sec to 2 min.) Blot dry with bibulous paper Examine your slide under the microscope. Record sketches of the organisms, size, color, morphology, and culture identification.

G+ G-