Conference on the Endocytosis

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Presentation transcript:

Conference on the Endocytosis

TRANSPORT OF MACROMOLECULES: ENDOCYTOSIS FLUID (OR BULK) FLOWS RECEPTOR-MEDIATED ENDOCYTOSIS SPECIFIC RECEPTOR COATES PITS (CLATHRIN) LYSOSOMAL FUSION WITH ENDOSOMES -- Recycling of receptors

Conference on Endocytosis: Receptor-mediated endocytosis Objectives: To reinforce the interplay among receptors, cargo, and actions of the apical portion of the cell by considering defects in the interplay To use techniques we have discussed to analyze the process of endocytosis To reinforce the idea that each step of a process is an opportunity for a disease

Insulin receptors do not recycle clathrin coated vesicle (dots label clathrin coat) uncoated vesicle (no dots)

Insulin receptors do not recycle clathrin coated vesicle (dots label clathrin coat) uncoated vesicle (no dots)

MANNOSE-6-PHOSPHTAE = SIGNAL FOR SORTING ENZYME TO LYSOSOME VIA RECEPTORS

TRANSCYTOSIS method to get antibodies into secretions or body fluids like milk, nasal fluid, or tears

EM 4c Intestinal absorptive cell Cut

Considerations Which cellular components are involved in receptor-mediated endocytosis? What materials are required to double label cellular components? Why do receptors recycle and why do some types of receptors not recycle? How does pH influence receptor-cargo binding?

Conference on Endocytosis: Receptor-mediated endocytosis Objectives: To reinforce the interplay among receptors, cargo, and actions of the apical portion of the cell by considering defects in the interplay To use techniques we have discussed to analyze the process of endocytosis To reinforce the idea that each step of a process is an opportunity for a disease