Invest. Ophthalmol. Vis. Sci ;52(6): doi: /iovs Figure Legend:

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From: Impaired Epithelial Wound Healing and EGFR Signaling Pathways in the Corneas of Diabetic Rats Invest. Ophthalmol. Vis. Sci.. 2011;52(6):3301-3308. doi:10.1167/iovs.10-5670 Figure Legend: Weakening of the EGFR-PI3K-AKT and ERK signaling pathways in diabetic rat corneal epithelial cells. Rat corneal epithelial cells, pooled from four corneas for each sample, were collected 48 hours after wounding (W48) with nonwounded (NW) as the control in normal (NL) or DM corneas, lysed in RIPA buffer, and processed for Western blot analysis. Equal amounts of protein (30 μg) were subjected to immunoblot using antibodies against phospho-EGFR (Tyr1068), pAKT (Ser473), pERK, or pBAD (Ser136 and Ser112). After they were stripped, the same membranes were reprobed with an antibody against the corresponding total protein. The blots were grouped as (A) EGFR, (B) PI3K-AKT, and (C) MAPK-ERK signaling pathways. Date of download: 1/1/2018 The Association for Research in Vision and Ophthalmology Copyright © 2018. All rights reserved.