From: Diffusion Tensor Imaging Detected Optic Nerve Injury Correlates with Decreased Compound Action Potentials after Murine Retinal Ischemia Invest. Ophthalmol.

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From: Diffusion Tensor Imaging Detected Optic Nerve Injury Correlates with Decreased Compound Action Potentials after Murine Retinal Ischemia Invest. Ophthalmol. Vis. Sci.. 2012;53(1):136-142. doi:10.1167/iovs.11-7908 Figure Legend: Immunohistochemistry revealed changes of intact axons staining with SMI-31 (a), injured axons with SMI-32 (b), total axon content with SMI-312 (c), and myelinated axons using MBP (d). With the progress of the injury, there were decreased numbers of intact axons labeled by SMI-31 in 3 and 7DPI compared with the control group (a, P < 0.005). Injured axons in the retina ischemia optic nerves were labeled with SMI-32. In the control optic nerve, no positive SMI-32 staining was seen, suggesting no injured axons in the control optic nerve. However, SMI-32 staining increased in 7DPI compared with that at 3DPI (b, P < 0.005). The total neurofilaments labeled by SMI-312 in 3DPI optic nerves were significantly decreased compared with control optic nerves (c, P < 0.005). The total neurofilaments at 7DPI are comparable to those at 3DPI. MBP staining in 3DPI was comparable with that in controls and were significantly higher than those at 7DPI (d, P < 0.05). Scale bar: 10 μm. Date of download: 12/27/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.