Denaturing High-Performance Liquid Chromatography

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Presentation transcript:

Denaturing High-Performance Liquid Chromatography Giovanni Roti, Roberto Rosati, Rossella Bonasso, Paolo Gorello, Daniela Diverio, Massimo Fabrizio Martelli, Brunangelo Falini, Cristina Mecucci The Journal of Molecular Diagnostics Volume 8, Issue 2, Pages 254-259 (May 2006) DOI: 10.2353/jmoldx.2006.050098 Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Chromatograms obtained with DHPLC technology. A: Colored lines show wild-type AML NPMc−. B: Reproducibility of the mutation A chromatograms in nine different cases. C: Mutations B and D with one different nucleotide. Violet, mutation B; green, mutation D. AML, acute myeloid leukemia; NPMc−, no nucleophosmin cytoplasmic delocalization/no NPM1 gene mutations; NPMc+, nucleophosmin cytoplasmic delocalization/NPM1 gene mutations. The Journal of Molecular Diagnostics 2006 8, 254-259DOI: (10.2353/jmoldx.2006.050098) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Nucleotide and protein sequence mutations of nucleophosmin (NPM1). Top rows indicate amino acids in single-letter codes, and bottom rows represent the corresponding codons. The most common site of insertion mutations (position 959) is indicated. Bold red letters are inserted nucleotides; yellow boxes are conserved tryptophan residues; green boxes are nucleolar exporting signal (NES). *New NPM mutations unraveled by DHPLC. The Journal of Molecular Diagnostics 2006 8, 254-259DOI: (10.2353/jmoldx.2006.050098) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 New mutations detected by DHPLC technology. Chromatograms were compared to wild-type (broken line) in all cases (A-D) and to known mutations in three of four cases (A-C). A: Dark green line: mutation B; light green line: [ins964_965(CTCT); 965G>T;966G>T; 967A>C; 968G>T; 969G>A]. B: Dark green line: mutation D; black line: [ins963-964(AGGA)]. C: Dark green line: mutation D; light green line: mutation [ins964_965(GCTT); 965G>C; 967>A>C; 968>G>C; 969G>C]. D: Brown line: mutation [ins959_960(GCCA)]. The Journal of Molecular Diagnostics 2006 8, 254-259DOI: (10.2353/jmoldx.2006.050098) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions