Volume 36, Issue 8, Pages (August 2015)

Slides:

Advertisements

Similar presentations

Volume 36, Issue 6, Pages (June 2015)

Advertisements

Alpha-Actinin 4 Is Associated with Cancer Cell Motility and Is a Potential Biomarker in Non–Small Cell Lung Cancer Ming-Chuan Wang, PhD, Ying-Hua Chang,

Nan-Hyung Kim, Ai-Young Lee Journal of Investigative Dermatology

Volume 2, Issue 8, Pages (August 2015)

Volume 35, Issue 3, Pages (March 2014)

Fig. 2 Effect of resistin and bFGF on human endothelial cells by MTS assay. A. Serum-starved HCAECs were treated with measured doses of resistin (0–80.

Volume 46, Pages (October 2016)

The effects of folic acid on global DNA methylation and colonosphere formation in colon cancer cell lines Nathan Farias, Nelson Ho, Stacey Butler, Leanne.

Aldehyde Dehydrogenase 1A1 Possesses Stem-Like Properties and Predicts Lung Cancer Patient Outcome Xiao Li, MD, Liyan Wan, MD, Jian Geng, MD, Chin-Lee.

Volume 355, Issue 2, Pages (December 2014)

Journal of Molecular and Cellular Cardiology

Gonadotropin-releasing hormone and gonadotropin-releasing hormone receptor are expressed at tubal ectopic pregnancy implantation sites Bo Peng, Ph.D.,

L. Nikitina, F. Wenger, M. Baumann, D. Surbek, M. Körner, C. Albrecht

Volume 15, Issue 6, Pages (June 2009)

MicroRNA-558 regulates the expression of cyclooxygenase-2 and IL-1β-induced catabolic effects in human articular chondrocytes S.J. Park, E.J. Cheon,

Selective interference of mTORC1/RAPTOR protects against human disc cellular apoptosis, senescence, and extracellular matrix catabolism with Akt and autophagy.

Integrin αvβ6 Promotes Lung Cancer Proliferation and Metastasis through Upregulation of IL-8–Mediated MAPK/ERK Signaling Pengwei Yan, Huanfeng Zhu, Li.

Volume 8, Issue 5, Pages (May 2017)

Expression of GRIM-19 in missed abortion and possible pathogenesis

MicroRNA-92a-3p regulates the expression of cartilage-specific genes by directly targeting histone deacetylase 2 in chondrogenesis and degradation G.

Requirement of the NF-κB pathway for induction of Wnt-5A by interleukin-1β in condylar chondrocytes of the temporomandibular joint: functional crosstalk.

MicroRNA-92a-3p regulates the expression of cartilage-specific genes by directly targeting histone deacetylase 2 in chondrogenesis and degradation G.

M. -H. Moon, J. -K. Jeong, Y. -J. Lee, J. -W. Seol, C. J. Jackson, S

Droxinostat, a Histone Deacetylase Inhibitor, Induces Apoptosis in Hepatocellular Carcinoma Cell Lines via Activation of the Mitochondrial Pathway and.

Impaired glycolytic metabolism causes chondrocyte hypertrophy-like changes via promotion of phospho-Smad1/5/8 translocation into nucleus T. Nishida,

Intermittent cyclic mechanical tension promotes endplate cartilage degeneration via canonical Wnt signaling pathway and E-cadherin/β-catenin complex cross-talk

Fibroblast Growth Factor 23 drives MMP13 expression in human osteoarthritic chondrocytes in a Klotho-independent manner A. Bianchi, M. Guibert, F. Cailotto,

X. Zhang, I. Prasadam, W. Fang, R. Crawford, Y. Xiao

NF-κBp65-specific siRNA inhibits expression of genes of COX-2, NOS-2 and MMP-9 in rat IL-1β-induced and TNF-α-induced chondrocytes Dr C. Lianxu, Ph.D.,

Decreased expression of Wiskott–Aldrich syndrome protein family verprolin-homologous protein 2 may be involved in the development of pre-eclampsia Juan.

TIEG1 Represses Smad7-Mediated Activation of TGF-β1/Smad Signaling in Keloid Pathogenesis Zhi-Cheng Hu, Fen Shi, Peng Liu, Jian Zhang, Dong Guo, Xiao-Ling.

MicroRNA-558 regulates the expression of cyclooxygenase-2 and IL-1β-induced catabolic effects in human articular chondrocytes S.J. Park, E.J. Cheon,

R. Piva, E. Lambertini, C. Manferdini, C. Capanni, L. Penolazzi, E

Reduction of CRKL expression in patients with partial DiGeorge syndrome is associated with impairment of T-cell functions Mauro Giacomelli, PhD, Rajesh.

Aldehyde Dehydrogenase 1A1 Possesses Stem-Like Properties and Predicts Lung Cancer Patient Outcome Xiao Li, MD, Liyan Wan, MD, Jian Geng, MD, Chin-Lee.

Expression and Function of RIG-I in Oral Keratinocytes and Fibroblasts

Alpha-Actinin 4 Is Associated with Cancer Cell Motility and Is a Potential Biomarker in Non–Small Cell Lung Cancer Ming-Chuan Wang, PhD, Ying-Hua Chang,

Volume 22, Issue 4, Pages (October 2015)

MicroRNA-320 regulates matrix metalloproteinase-13 expression in chondrogenesis and interleukin-1β-induced chondrocyte responses F. Meng, Z. Zhang, W.

Volume 11, Issue 6, Pages (June 2012)

Fas ligand+ fallopian tube epithelium induces apoptosis in both Fas receptor+ T lymphocytes and endometrial cells Sebastian E. Illanes, M.D., Kevin Maisey,

X. Luo, H. Zhao, L. Hennah, J. Ning, J. Liu, H. Tu, D. Ma

Laminin γ2 Mediates Wnt5a-Induced Invasion of Gastric Cancer Cells

Bone morphogenetic protein 4 promotes mammalian oogonial stem cell differentiation via Smad1/5/8 signaling Eun-Sil Park, Ph.D., Dori C. Woods, Ph.D.,

CaMKII inhibition in human primary and pluripotent stem cell-derived chondrocytes modulates effects of TGFβ and BMP through SMAD signaling B. Saitta,

Volume 152, Issue 1, Pages (January 2019)

Simvastatin Protects Human Melanocytes from H2O2-Induced Oxidative Stress by Activating Nrf2 Yuqian Chang, Shuli Li, Weinan Guo, Yuqi Yang, Weigang Zhang,

Suppression of Sestrins in aging and osteoarthritic cartilage: dysfunction of an important stress defense mechanism T. Shen, O. Alvarez-Garcia, Y. Li,

TWEAK/Fn14 Signals Mediate Burn Wound Repair

Volume 137, Issue 1, Pages (July 2009)

Volume 70, Issue 9, Pages (November 2006)

EB3 Regulates Microtubule Dynamics at the Cell Cortex and Is Required for Myoblast Elongation and Fusion Anne Straube, Andreas Merdes Current Biology

Decreased expression of FOXA2 promotes eutopic endometrial cell proliferation and migration in patients with endometriosis Anping Lin, Juan Yin, Chao.

Molecular Therapy - Nucleic Acids

Volume 9, Issue 5, Pages (May 2009)

Regulation of mechanical stress-induced MMP-13 and ADAMTS-5 expression by RUNX-2 transcriptional factor in SW1353 chondrocyte-like cells T. Tetsunaga,

Increased Expression of Laminin Subunit Alpha 1 Chain by dCas9-VP160

Activin Signals through SMAD2/3 to Increase Photoreceptor Precursor Yield during Embryonic Stem Cell Differentiation Amy Q. Lu, Evgenya Y. Popova, Colin.

Volume 26, Issue 8, Pages (August 2018)

EVA1A/TMEM166 Regulates Embryonic Neurogenesis by Autophagy

Volume 44, Issue 4, Pages (April 2016)

Long Noncoding RNA BC as a Novel Therapeutic Target for Colorectal Cancer that Suppresses Metastasis by Upregulating TIMP3 Jiaxin Lin, Xin Tan,

Volume 25, Issue 10, Pages (October 2017)

Molecular Therapy - Nucleic Acids

Volume 6, Issue 2, Pages (February 2016)

Volume 26, Issue 10, Pages (October 2018)

A Trace Amount of Galactose, a Major Component of Milk Sugar, Allows Maturation of Glycoproteins during Sugar Starvation Norio Sasaoka, Hiromi Imamura,

Volume 23, Issue 10, Pages (June 2018)

Volume 21, Issue 5, Pages (May 2012)

Aminoglycoside Enhances the Delivery of Antisense Morpholino Oligonucleotides In Vitro and in mdx Mice Mingxing Wang, Bo Wu, Sapana N. Shah, Peijuan.

Presentation transcript:

Volume 36, Issue 8, Pages 809-820 (August 2015) Laminin α4 (LAMA4) expression promotes trophoblast cell invasion, migration, and angiogenesis, and is lowered in preeclamptic placentas N. Shan, X. Zhang, X. Xiao, H. Zhang, C. Tong, X. Luo, Y. Chen, X. Liu, N. Yin, Q. Deng, H. Qi Placenta Volume 36, Issue 8, Pages 809-820 (August 2015) DOI: 10.1016/j.placenta.2015.04.008 Copyright © 2015 Terms and Conditions

Fig. 1 Dynamic LAMA4 Expression in Placentas. (A) Immunohistochemistry (IHC) of LAMA4 in human villi. (B) CK7 expression in human villi. (C and I) Negative controls with nonimmune rabbit IgG. (D) LAMA4 strongly expressed in human decidua in the first trimester. (E) HLA-G expression from the identical sample of human decidua. (F) CK7 expression in human decidua. (G) LAMA4 expression was moderate in the term placenta. (H) LAMA4 was rarely expressed in the preeclamptic placentas. Scale bar = 100 μm. Abbreviations: CTB, cytotrophoblasts; STB, syncytiotrophoblasts; EVT, extravillous trophoblasts, PE, preeclamptic. Placenta 2015 36, 809-820DOI: (10.1016/j.placenta.2015.04.008) Copyright © 2015 Terms and Conditions

Fig. 2 LAMA4 Expression in Placentas and Maternal Serum. (A, B) LAMA4 expression was decreased in placentas of preeclamptics (N = 22) compared to healthy controls (N = 20) determined by Western blotting (n = 3, p < 0.01) and real-time RT-PCR (n = 3, p < 0.01). (C) LAMA4 expression in the maternal serum was not significantly different between preeclamptics (N = 20) and healthy controls (N = 20, p > 0.05). Abbreviations: PE, preeclamptic. Placenta 2015 36, 809-820DOI: (10.1016/j.placenta.2015.04.008) Copyright © 2015 Terms and Conditions

Fig. 3 Expression of LAMA4, MMP2, MMP9, TIMP1, and TIMP2 after Various Treatments. (A) Expression of LAMA4, MMP2, MMP9, TIMP1, and TIMP2 were examined by Western blotting. (B, C) LAMA4 expression was decreased after various interventions as compared to the control group according to Western blotting and real-time RT-PCR. (D–G) Representative immunoblotting of MMP2, MMP9, TIMP1, and TIMP2 normalized to β-actin and comparisons of protein concentrations after various treatments. Placenta 2015 36, 809-820DOI: (10.1016/j.placenta.2015.04.008) Copyright © 2015 Terms and Conditions

Fig. 4 Invasive and Migratory Abilities of HTR8/SVneo Cells Decreased with LAMA4 Silencing and H/R Conditions. (A, B) The invasive and migratory abilities of HTR8/SVneo cells decreased after exposure to H/R conditions and LAMA4 silencing (both n = 3, p < 0.001). (C, D) Statistical bar graphs of the invasion and migration assays. Data were analyzed by analysis of variance (ANOVA) to assess significant differences. Original images were taken at 200× magnification. Placenta 2015 36, 809-820DOI: (10.1016/j.placenta.2015.04.008) Copyright © 2015 Terms and Conditions

Fig. 5 Effects of Different Treatments on HTR8/SVneo Cell Viability and Apoptosis. (A) Flow cytometric analysis of apoptosis across different treatments, including normal controls, H/R, LAMA4 silencing, and SB. (B) MTT assay showed no significant differences in viability after LAMA4 silencing and SB treatment by analysis of variance (ANOVA). (C) Significant differences in cell apoptosis were observed after H/R treatment (n = 3, p < 0.001). Placenta 2015 36, 809-820DOI: (10.1016/j.placenta.2015.04.008) Copyright © 2015 Terms and Conditions

Fig. 6 Tube Formation of HTR8/SVneo Cells under Different Treatments. (A) Nontreated group. (B) siLAMA4 group. (C) H/R group. (D) SB group. Arrows indicate capillary-like structures. Bars = 100 μm. (E) Bar graphs showing quantification in the tube formation assay. The tube formation index was expressed as tube perimeter (mm) per area (mm [2]). **p < 0.01 compared with the nontreated control group. Placenta 2015 36, 809-820DOI: (10.1016/j.placenta.2015.04.008) Copyright © 2015 Terms and Conditions

Fig. 7 Immunofluorescent Detection of LAMA4 Proteins and Nuclei in HTR8/SVneo Cells under Different Treatments. (A–C) LAMA4 expression (green) in normal control cells. (D–F) LAMA4 expression (green) decreased after siRNA transfection. (G–I) LAMA4 expression (green) decreased after 48 h of H/R. (J–L) LAMA4 expression (green) was not changed after SB treatment. Cell nuclei stained by propidium iodide (red) (n = 3 in triple). Original images were taken at 400× magnification. Placenta 2015 36, 809-820DOI: (10.1016/j.placenta.2015.04.008) Copyright © 2015 Terms and Conditions

Fig. 8 Placental Villous Explants after 72-h Culture In Vitro. (A) Nontreated group. (B) siLAMA4 group. (C) H/R group. (D) SB group. Bars = 100 μm. (E) Graphical representation of trophoblast cell migration distance in placental villous explants (n = 5). **p < 0.01 compared with nontreated control group. Placenta 2015 36, 809-820DOI: (10.1016/j.placenta.2015.04.008) Copyright © 2015 Terms and Conditions

Fig. 9 MAPK Signaling Pathway under Different Treatments. (A) Protein expressions of p-p38, p-JNK, and p-ERK were examined by Western blotting. (B–D) Representative immunoblotting of p-p38, p-JNK, and p-ERK normalized with β-actin and comparisons of protein concentrations after various treatments. Placenta 2015 36, 809-820DOI: (10.1016/j.placenta.2015.04.008) Copyright © 2015 Terms and Conditions

Fig. 10 DNA Methylation of Placental LAMA4. CpG sites from +5312 to +5669 bps (relative to the transcription start site) of LAMA4 were included in the bisulfite genomic sequencing assay. A total of 33 CpG sites in the normal and preeclamptic (PE) placentas were included, respectively. Each square represents an individual CpG site with the yellow portions representing methylation-positive results and the blue portions representing methylation-negative results. The sizes of the yellow portions indicate fractional methylation. The data are presented as the mean values from the placental samples. Placenta 2015 36, 809-820DOI: (10.1016/j.placenta.2015.04.008) Copyright © 2015 Terms and Conditions

Supplement 1 Invasive and Migratory Abilities of HTR8/SVneo Cells Decreased Under the H/R Conditions. The invasive, migratory, and tube formation abilities of HTR8/SVneo cells decreased after exposure to H/R conditions (n = 3 for both groups, p < 0.01). Bar graphs displaying data from the invasion, migration, and tube formation assays. Data were analyzed by independent t-tests to assess significant differences. Original images were taken at 200× magnification. Placenta 2015 36, 809-820DOI: (10.1016/j.placenta.2015.04.008) Copyright © 2015 Terms and Conditions