Some organisms have a CELL WALL Plants (cellulose) Algae (polysaccharide) Fungi (chitin) Prokaryotes(peptidoglycan)

What does the cell wall do? Scaffolding - mechanical support and structure Protection Involved in Cell-cell communication Maintenance of structure (turgor)

Movement across the cell membrane

Where is the water in your body?

Where are membranes located? Cell membrane (double membrane) Organelles (membrane-bound) can be single or double membrane) Secret Universe Introduction to cell membrane

What do membranes do? All cells live in an aqueous environment (surrounded by water, ions and molecules) …they must control what gets in (nutrients) and out ( waste products and signalling molecules) they need to communicate with each other… How is this achieved?....

What do membranes do? Protective barrier Cell-Cell signalling Transport of nutrients, products and waste products Localisation of function within organelles Cell membrane function

Membranes are: Semi-permeable: controls entry and exit of substances Self-sealing Flexible, mobile fluid mosaics

Let’s meet the components of the cell membrane

Phospholipid bilayer polar hydrophilic heads nonpolar hydrophobic tails polar hydrophilic heads

Substances can be hydrophilic or hydrophobic

Membrane Proteins Each membrane has its own unique set of proteins Proteins act as: CHANNELS to get substances in or out of the cell or organelle PUMPS to get substances in or out of the cell or organelle RECEPTORS to allow the cell or organelle to respond to a stimulus

3.4.2 Explain the importance of the membrane proteins to transport across the membrane

Protein channels move substances from one side of membrane to the other

Protein pumps move substances from one side of membrane to the other

Protein receptors

Membranes have sugars (carbohydrates) attached to their surfaces ‘Chemical identification cards’ Used for cell communication Used by the immune system to identify self or ‘non-self’ (invaders) The four human blood groups (A, B, AB, and O) differ in the external carbohydrates on red blood cells.

Blood cells have glycoproteins (blood type)

Let’s Review… Let's build a membrane from scratch… And now…let’s make a membrane!

Movement across the Cell Membrane

How do things get into and out of our cells? PASSIVE MECHANISMS These don’t require energy Simple diffusion Facilitated diffusion Osmosis ACTIVE MECHANISMS These require energy to transport substances (often against their concentration gradient) Protein pumps Endocytosis/ exocytosis

3.5.4 State that the energy for diffusion comes from the kinetic energy of random movement of molecules and ions Particle theory states that all matter consists of many, very small particles, which are constantly moving, or in a continual state of motion. The degree to which the particles move is determined by the amount of energy they have and their relationship to other particles

3.4.2 Explain the importance of the membrane proteins to transport across the membrane

3.5.1: Diffusion: Definition Diffusion is the net movement of particles from a region of their higher concentration to a region of their lower concentration down a concentration gradient, as a result of their random movement Works for small particles

Simple Diffusion 2nd Law of Thermodynamics governs biological systems: the universe tends towards disorder (entropy) Movement from high concentration of that substance to low concentration of that substance. Diffusion: movement of small, soluble particles from high  low concentration

Diffusion Movement is from HIGH to LOW concentration movement of water “passive transport” no energy needed movement of water diffusion osmosis

3.5.3 substances move into and out of cells by diffusion through the cell membrane; this is a dynamic process (always happening) Gas exchange in the lungs Gas exchange in plants

Gas exchange between lungs and blood is via diffusion

Gas exchange in plants is via diffusion

3.4.2 Explain the importance of the membrane proteins to transport across the membrane

Facilitated Diffusion Facilitated diffusion is diffusion of specific molecules through protein channels in the cell membrane no energy is required facilitated = with help open channel = fast transport high low Donuts! Each transport protein is specific as to the substances that it will translocate (move). For example, the glucose transport protein in the liver will carry glucose from the blood to the cytoplasm, but not fructose, its structural isomer. Some transport proteins have a hydrophilic channel that certain molecules or ions can use as a tunnel through the membrane -- simply provide corridors allowing a specific molecule or ion to cross the membrane. These channel proteins allow fast transport. For example, water channel proteins, aquaporins, facilitate massive amounts of diffusion.

Factors affecting diffusion Concentration gradient Temperature Surface area for diffusion Distance for diffusion (size of particle) (charged or uncharged)

Concentration gradient Temperature Surface area for diffusion Factors affecting the rate of facilitated diffusion are the same as for simple diffusion Concentration gradient Temperature Surface area for diffusion Distance for diffusion

Osmosis water diffuses through partially permeable membranes from higher to lower concentrations by osmosis water moves in and out of cells by osmosis through the cell membrane

Osmosis: Definition Osmosis is the net movement of water molecules from a region of higher water concentration [potential] (dilute solution) to a region of lower water concentration [potential] (concentrated solution), through a partially permeable membrane Like other types of diffusion, osmosis is a dynamic process [achieves balance (equilibrium) but never stops]

Movement of water across membranes involves BOTH simple AND facilitated diffusion

Factors affecting osmosis Concentration gradient Temperature Surface area for diffusion Distance for diffusion

Comparing ‘water concentration’ of different solutions Direction of osmosis is determined by comparing total solute concentrations on either side of the membrane: Hypertonic - more solute, less water Hypotonic - less solute, more water Isotonic - equal solute, equal water hypotonic hypertonic water net movement of water

freshwater balanced saltwater

Osmosis is the diffusion of water across a semi-permeable membrane Facilitated diffusion of water from high concentration of water to low concentration of water across a semi-permeable membrane

Examples of Osmosis in Biology Absorption of water by plant roots. Re-absorption of water in the kidney. Re-absorption of tissue fluid into blood capillaries. Absorption of water in the GI tract

Water moves across a membrane from the hypotonic solution to the hypertonic solution Animation of osmosis 1 Animation of osmosis 2: why water balance matters

How do things get into and out of our cells? PASSIVE MECHANISMS These don’t require energy Simple diffusion Facilitated diffusion Osmosis ACTIVE MECHANISMS These require energy to transport substances (often against their concentration gradient) Protein pumps Endocytosis/ exocytosis

Active transport 1: Protein pumps Active transport uses energy (ATP) to transport substances AGAINST a concentration gradient into/out of the cell The energy is used to change the shape of the ‘protein pump’ and thus import/export specific molecule Animation protein pumps in plants low high ATP

3.6.2 Discuss the importance of active transport as a process for movement across membranes: e.g. ion uptake by root hairs and uptake of glucose by epithelial cells of villi and kidney tubules

3.6.3 Explain how protein molecules move particles across a membrane during active transport

Transport summary simple diffusion facilitated diffusion ATP active transport

Active Mechanisms: Endocytosis and Exocytosis A simple one... Animation Animation 2 Endocytosis/ exocytosis are import/export of materials by infolding/outfolding of the cell membrane

Cell responses 3.7 3.7.1 Explain the effects on plant tissues of immersing them in solutions of different concentrations by using the terms turgid, turgor pressure, plasmolysis and flaccid

3.7.3 Explain the importance of osmosis on animal cells and tissues

3.7.4 Explain how plants are supported by the turgor pressure within cells, in terms of water pressure acting against an inelastic cell wall