FLOWCYTOMETRY

What Is Flowcytometry? Flow→ Fluid Cyto→ Cell Metry→ Measurement

It allows simultaneous multi-parameter analysis of single cells - Cell Size - Granularity - Cell Surface and Intracellular Molecules - Cell Proliferation & Apoptosis - DNA Analysis

How Does Flowcytometry Work? - Fluidics - Optics - Electronics

Fluidics When the stained cell sample in suspension buffer is run through the cytometer, the tiny “stream” of fluid takes the cells past the laser light one cell at a time.

Optics Lasers producing light at specific wavelength Scatter

Forward Scatter = Size of The Particle

Optics

Side Scatter = Granularity of The Cell

For Surface and Intracellular molecules Antibody Lable

Fluorescent Compound or Fluorochrome Labeling of the antibody Fluorescent Compound or Fluorochrome

Labeling of the antibody Light Fluorochrome Excitation Emission

Fluorochromes Surface and Intracellular Staining

Optics

Data Analysis

Good luck