E. Homsi, P. Janino, J.B.L. de Faria Kidney International

Slides:

Advertisements

Similar presentations

Volume 61, Issue 4, Pages (April 2002)

Advertisements

Volume 66, Issue 6, Pages (December 2004)

Volume 67, Issue 5, Pages (May 2005)

Volume 79, Issue 1, Pages (January 2011)

Volume 70, Issue 4, Pages (August 2006)

Stem cells for kidney repair: useful tool for acute renal failure?

Testosterone promotes apoptotic damage in human renal tubular cells

The Case ∣ Acute renal failure and anemia

Volume 69, Issue 12, Pages (June 2006)

Volume 61, Issue 2, (February 2002)

Volume 66, Issue 6, Pages (December 2004)

The Case ∣ Generalized petechiae and acute renal failure

Volume 69, Issue 8, Pages (April 2006)

C.-W. Tsai, V.-C. Wu, W.-C. Lin, J.-W. Huang, M.-S. Wu

Epidermal growth factor receptor inhibition attenuates early kidney enlargement in experimental diabetes Lesley Wassef, Darren J. Kelly, Richard E. Gilbert

Cytochrome P450 2E1 null mice provide novel protection against cisplatin-induced nephrotoxicity and apoptosis Hua Liu, Radhakrishna Baliga Kidney International

Volume 63, Issue 4, Pages (April 2003)

Volume 61, Issue 1, Pages (January 2002)

Hua Liu, Steven A. Bigler, Jeffrey R. Henegar, Radhakrishna Baliga

Volume 79, Issue 1, Pages (January 2011)

Volume 60, Issue 3, Pages (September 2001)

Vitamin E attenuates crystal formation in rat kidneys: Roles of renal tubular cell death and crystallization inhibitors H.-S. Huang, J. Chen, C.-F. Chen,

Volume 70, Issue 2, Pages (July 2006)

Volume 73, Issue 4, Pages (February 2008)

Volume 70, Issue 7, Pages (October 2006)

Volume 82, Issue 9, Pages (November 2012)

Volume 63, Issue 2, Pages (February 2003)

Volume 71, Issue 8, Pages (April 2007)

P.-A. Hueber, P. Waters, P. Clarke, M. Eccles, P. Goodyer

The renal lesions that develop in neonatal mice during angiotensin inhibition mimic obstructive nephropathy Yoichi Miyazaki, Shinya Tsuchida, Agnes Fogo,

Volume 67, Issue 6, Pages (June 2005)

Role of meprin A in renal tubular epithelial cell injury

IL-18 is expressed in the intercalated cell of human kidney

Richard A. Zager, Ali C.M. Johnson, Sherry Y. Hanson

Volume 70, Issue 11, Pages (December 2006)

Volume 66, Issue 6, Pages (December 2004)

Volume 60, Issue 6, Pages (December 2001)

Volume 83, Issue 3, Pages (March 2013)

Volume 66, Issue 2, Pages (August 2004)

Volume 63, Issue 6, Pages (June 2003)

Volume 61, Issue 1, Pages (January 2002)

Volume 72, Issue 5, Pages (September 2007)

Volume 56, Issue 3, Pages (September 1999)

Volume 63, Issue 5, Pages (May 2003)

Volume 61, Issue 4, Pages (April 2002)

An unusual cause of acute renal failure

The pathological role of Bax in cisplatin nephrotoxicity

Cytokine cooperation in renal tubular cell injury: The role of TWEAK

Volume 60, Issue 3, Pages (September 2001)

Volume 72, Issue 8, Pages (October 2007)

Volume 54, Issue 4, Pages (October 1998)

Peroxisome proliferator-activated receptor-gamma agonist is protective in podocyte injury-associated sclerosis H.-C. Yang, L.-J. Ma, J. Ma, A.B. Fogo

Volume 69, Issue 12, Pages (June 2006)

Volume 69, Issue 3, Pages (February 2006)

Distribution of postsynaptic density proteins in rat kidney: Relationship to neuronal nitric oxide synthase Akihiro Tojo, David S. Bredt, Christopher.

Volume 73, Issue 3, Pages (February 2008)

Protective actions of administered mesenchymal stem cells in acute kidney injury: relevance to clinical trials Christof Westenfelder, Florian E. Togel

MEK inhibitor, U0126, attenuates cisplatin-induced renal injury by decreasing inflammation and apoptosis Sang-Kyung Jo, Won Yong Cho, Su Ah Sung, Hyoung.

Volume 64, Pages S52-S56 (October 2003)

Organ transplantation goes to the movies

Volume 56, Issue 6, Pages (December 1999)

Delayed administration of darbepoetin or erythropoietin protects against ischemic acute renal injury and failure D.W. Johnson, B. Pat, D.A. Vesey, Z.

Volume 70, Issue 3, Pages (August 2006)

Volume 70, Issue 10, Pages (November 2006)

Volume 72, Issue 11, Pages (December 2007)

Cell cycle regulation: Repair and regeneration in acute renal failure

E. Homsi, P. Janino, J.B.L. de Faria Kidney International

T cells and T-cell receptors in acute renal failure

D.J. Askenazi, D.I. Feig, N.M. Graham, S. Hui-Stickle, S.L. Goldstein

Volume 70, Issue 1, Pages (July 2006)

Presentation transcript:

Role of caspases on cell death, inflammation, and cell cycle in glycerol-induced acute renal failure E. Homsi, P. Janino, J.B.L. de Faria Kidney International Volume 69, Issue 8, Pages 1385-1392 (April 2006) DOI: 10.1038/sj.ki.5000315 Copyright © 2006 International Society of Nephrology Terms and Conditions

Figure 1 Evaluation of acute tubular necrosis (ATN) in Gly-ARF 5 ml/kg at 24 h. The pan-caspase inhibitor (Z-VAD-FMK) treated rats had less ATN than untreated rats. *P<0.05 vs Gly-ARF. Kidney International 2006 69, 1385-1392DOI: (10.1038/sj.ki.5000315) Copyright © 2006 International Society of Nephrology Terms and Conditions

Figure 2 Evaluation of tubular cell apoptosis (TUNEL positive cells) in Gly-ARF 5 ml/kg. (a) Number of TUNEL positive cells significantly increased in the renal medulla 6 h after Gly-ARF induction. N: 5 rats/group. (b) Separate experiment showing that Z-VAD-FMK significantly reduced the number of TUNEL positive cells 6 h after Gly-ARF 5 ml/kg. N: 5 rats/group. *P<0.05 vs other groups. Kidney International 2006 69, 1385-1392DOI: (10.1038/sj.ki.5000315) Copyright © 2006 International Society of Nephrology Terms and Conditions

Figure 3 Evaluation of immunohistochemistry for IL-1β and IL-18 in the renal parenchyma. IL-1β immunostaining observed at low power field (× 40) in (a) control rat and (b) Gly-ARF. IL-1β expression predominates in the renal cortex and is enhanced in Gly-ARF. At higher magnification (× 400), IL-1β expression in (c) control rat and (d) Gly-ARF showing tubule cells positivity. The expression of IL-18 in (e) control rat and (f) Gly-ARF were similar and observed in the cortex and outer medulla tubule cells. Kidney International 2006 69, 1385-1392DOI: (10.1038/sj.ki.5000315) Copyright © 2006 International Society of Nephrology Terms and Conditions

Figure 4 Western blot from cortical kidney homogenates showing the expression of IL-1β and IL-18. (a) The IL-1β inactive 35 kDa precursor was detected in controls (C), Gly-ARF (Gly), and Gly-ARF treated with Z-VAD-FMK (Gly+Z-VAD-FMK), whereas the 17 kDa active IL-1β form was detected in Gly-ARF and absent in Gly-ARF+Z-VAD-FMK. (b) The IL-18 inactive 24 kDa precursor was detected in controls (C), Gly-ARF (Gly), and Gly-ARF treated with Z-VAD-FMK (Gly+Z-VAD-FMK), whereas the 18 kDa active IL-18 form was detected in control and Gly-ARF and absent in Gly-ARF+Z-VAD-FMK. Kidney International 2006 69, 1385-1392DOI: (10.1038/sj.ki.5000315) Copyright © 2006 International Society of Nephrology Terms and Conditions

Figure 5 Number of BrdU positive cells in renal parenchyma 48 h after Gly-ARF. There was a significant increase in BrdU positive cell (mitosis) in Gly-ARF. Z-VAD-FMK treated rats showed positivity similar to untreated Gly-ARF. N: 6 rats/group, *P<0.001 vs control. Kidney International 2006 69, 1385-1392DOI: (10.1038/sj.ki.5000315) Copyright © 2006 International Society of Nephrology Terms and Conditions

Figure 6 Western blot from cortical kidney homogenates showing expression of p27Kip detected in controls (c) and absent in Gly-ARF (Gly) or Gly-ARF treated with Z-VAD-FMK (Gly+Z-VAD-FMK). Kidney International 2006 69, 1385-1392DOI: (10.1038/sj.ki.5000315) Copyright © 2006 International Society of Nephrology Terms and Conditions